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Iranian Journal of Biotechnology، جلد ۵، شماره ۲، صفحات ۱۱۴-۱۱۸
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عنوان انگلیسی Biosorption of Textile Dyes and Effluents by Pleurotusflorida and Trametes Hirsutawith Evaluation of Their Laccase Activity
چکیده انگلیسی مقاله The rate and efficiency of decolorization of dyes like Blue CA, Black B133, and Corazol Violet SR were tested to evaluate white rot fungal strains. Trametes hirsuta and Pleurotus florida showed the greatest extent of decolorization on nutrient salt media. Maximum decolorization of 200 mg/l of Blue 133 was obtained by 4 days old incubated Pleurotus florida followed by Trametes hirsuta after 6 days. An attempt was made to improve the decolorization activity of both organisms with different concentrations of glucose 1 and 2% (w/v). The decolorization activity may be due to the laccase enzyme of white rot fungi. The production of this enzyme was estimated using solid state fermentation with rice bran as a substrate. It was found that P. florida exhibited 0.175 U/ml of laccase activity followed 0.126U/ml by T. hirsute, respectively. Decolourization was found to be more effective with P. florida in the presence of 2% (w/v) glucose. Crude extract containing the laccase enzyme was isolated and confirmed by SDS PAGE
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نویسندگان مقاله sathiya moorthi perumal | sathiya moorthi
department of industrial biotechnology, dr. m.g.r. educational and research institute, dr. m.g.r. university, chennai-600 095, india

deecaraman munuswamy |
department of industrial biotechnology, dr. m.g.r. educational and research institute, dr. m.g.r. university, chennai-600 095, india

periyar selvam sellamuthu | periyar selvam
center for advanced studies in botany, university of madras, chennai-600 025, india

murugesan kandasamy |
center for advanced studies in botany, university of madras, chennai-600 025, india

kalaichelvan puthupalayam thangavelu | puthupalayam thangavelu
center for advanced studies in botany, university of madras, chennai-600 025, india

نشانی اینترنتی http://www.ijbiotech.com/article_7030_668dfcef961b0e1f6af7f806ec70d6e5.pdf
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