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Iranian Journal of Biotechnology، جلد ۵، شماره ۱، صفحات ۴۲-۴۷
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عنوان انگلیسی Detection of Types 40 and 41 Adenoviruses in Stool Samples of Diarrheal Children by Solid Phase PCR
چکیده انگلیسی مقاله Adenoviruses (AdVs) types 40 and 41 are the causative agents of diarrhea in children. Hence, rapid sensitive and specific detection of these viruses are of clinical importance. The customary methods such as propagation of virus in cell culture suffer from limitations. Detection of immobilized amplified products in a one phase system (DIAPOPS) method has the potential to overcome these problems. A DIAPOPS method for detection of AdV types 40 and 41 was designed. Forward primers were covalently linked to the Nucleolink surface. After amplification of a 745 bp sequence of DNA binding protein gene, the amplified product was hybridized with the biotinylated probe. The hybrids were detected by the antibody-peroxidase conjugate. After optimization of the DIAPOPS conditions, 80 stool samples from children with clinical manifestation of viral diarrhea were tested. Their DIAPOPS results were compared with those of the conventional polymerase chain reaction (PCR) assay. Positive results were obtained in 11 samples. The comparison between conventional PCR and DIAPOPS showed a significant increase in sensitivity of the DIAPOPS test, 6 samples shown to be negative by conventional PCR, were demonstrated positive by DIAPOPS (p=0.00). The DIAPOPS assay presented in this study can provide a rapid, sensitive, specific and economic method for detection of viral infections. The assay can be performed for numerous samples simultaneously in a day. This DIAPOPS method can provide a practical and reliable tool for diagnosis of enteric adenoviruses. In addition, the risk of contamination in this assay is low.
کلیدواژه‌های انگلیسی مقاله DIAPOPS,PCR,Children diarrhea,Adenoviruses types 40 and 41
نویسندگان مقاله روح اله نخعی سیستانی | nakhaei sistani
department of genetics, faculty of basic sciences, tarbiat modares university, p.o. box14115-175, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

مجید صادقی زاده |
pouya zistech ltd. p.o. box 16315-769 tehran, ir iran and department of genetics, faculty of basic sciences, tarbiat modares university, p.o. box14115-175, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

حوریه صادری |
department of microbiology, faculty of medicine, shahed university, p.o. box 14155-7435, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

نرگس خرازانی تفرشی | kharazani tafreshi
pouya zistech ltd. p.o. box 16315-769 tehran, ir iran and department of genetics, faculty of basic sciences, tarbiat modares university, p.o. box14115-175, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

مهرداد بهمنش |
department of genetics, faculty of basic sciences, tarbiat modares university, p.o. box14115-175, tehran, ir iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

هادی شیرزاد |
department of biology, faculty of sciences, imam hossein university, p.o. box 15815-3539,tehran, ir iran
سازمان اصلی تایید شده: دانشگاه جامع امام حسین (Imam hosseyn university)

نشانی اینترنتی http://www.ijbiotech.com/article_7028_cba0bc233bc3c33408db55085824c5ad.pdf
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