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Iranian Journal of Fisheries Sciences، جلد ۱۴، شماره ۱، صفحات ۲۳۲-۲۴۵
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عنوان انگلیسی Pathogenicity of Aeromonas hydrophila in giant freshwater prawn Macrobrachium rosenbergii, cultured in East Malaysia
چکیده انگلیسی مقاله Aeromonas infections are becoming a major risk factor in commercial aquaculture and it has been reported that a wide variety of fish and shellfish species are susceptible to this infection. In this study, 3 isolates of Aeromonas hydrophila were isolated from giant freshwater prawn (Macrobrachium rosenbergii) cultured in Kuala Pilah Simbilan Nigri in East Malaysia. Conventional and rapid identification systems (API 20E strips) were used for preliminary identification based on the biochemical characters of the isolated bacteria. On the other hand, polymerase chain reaction (PCR) using the universal primer 16S rRNA, was done as an accurate and confirmatory identification. The virulence of A. hydrophila was determined using a pathogenicity test via I/M injection. The results revealed that the isolated bacteria were identified as A. hydrophila that revealed a high degree of similarity (98%) to the NCBI or Genbank database. Based on pathogenicity test results, LD50 was determined as 1×106 CFU/50µl, while 1×107 CFU/50µl induced 100% mortality in the experimentally injected prawns. Histopathological changes were found in several organs including gill, hepatopancreas and heart. Those changes were mainly, melanisation, tissue erosion and necrosis, infiltration and hyperplasia of gill lamellae and mild or massive haemocyte reaction in the infected organs.
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نویسندگان مقاله s عبدالنبی | s abdolnabi


محمدیوسف نیا salwany | m y ina salwany


حمیدرضا داوود | h m daud


سید دامون mariana | s d mariana


یوسف موسی عبدالهادی | y m abdelhadi


نشانی اینترنتی http://www.jifro.ir/browse.php?a_code=A-10-1272-146&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده fish disease
نوع مقاله منتشر شده پژوهشی
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