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Iranian Journal of Fisheries Sciences، جلد ۱۳، شماره ۱، صفحات ۱۶۸-۱۷۷
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عنوان انگلیسی Pathogenicity of viral nervous necrosis virus for Guppy fish, Poecilia reticulata
چکیده انگلیسی مقاله The pathogenicity of a Nervous Necrosis Virus isolate obtained from naturally infected Golden grey mullet (Liza auratus) suffering serious mortalities in Iranian coastline water of the Caspian Sea was investigated for first time. An experimental infection has been performed using three groups, two experimental groups and one control group of Guppy (Poecilia reticulata) with mean weight 0.47±0.09 g, at temperature 25ºC. The infectious dosage (50 ml) with TCID50/ml= 10 4.25for 2 hours in group 1and 4 hours in group 2 developed the disease with immersion method. Clear clinical signs associated with significant mortality were observed since 15 dpi. Cumulative mortalities rose to 100% at 30 dpi. While in the control group no mortality was recorded. Virus was re-isolated on SSN-1 cell line that showing typical CPE developed after inoculation with tissues filtrate from dead fish. Histopathological examination of exposed fish, showed clear vacuolization in the granular layer of the retina and cerebellum. TEM micrographs revealed intracytoplasmic vacuoles in the retina of infected Guppy. IHC revealed the presence of viral antigens in the brain and retina. These results confirmed the pathogenicity of the NNV isolate obtained from Golden grey mullet suffering high mortality with regard to suggest that the same agent isolated from golden grey mullet is very likely the cause of the mortality observed in the same species.
کلیدواژه‌های انگلیسی مقاله VNN, Golden grey mullet, Caspian Sea
نویسندگان مقاله a نظری | a nazari


م دیوید حسن | m d hassan


g bovo | g bovo


محمدجواد ذریه زهرا | m j zorriehzahra


t i عزمی | t i azmi


سید سعید ارشد | s s arshad


نشانی اینترنتی http://www.jifro.ir/browse.php?a_code=A-10-1272-71&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده fish disease
نوع مقاله منتشر شده پژوهشی
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