این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Public Health، جلد ۴۶، شماره ۷، صفحات ۹۴۸-۹۵۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی In Vitro Hb Production in Β-thalassemia Patients Is Not a Predictor of Clinical Responsiveness to Hydroxyurea
چکیده انگلیسی مقاله Background: The hematologic response to hydroxyurea (HU) is varied among β-thalassemia (BT) patients. The BCL11A and SOX6 genes are involved in response to HU. This study aimed to investigate the in-vitro responsiveness of HU among BT major patients homozygote for IVSII-1G>A mutation and XmnI single nucleotide polymorphism (SNP) in order to find whether the in-vitro Hb concentration is a predictor of clinical (HU) responsiveness. Methods: In this case-control study, twenty BT patients homozygote for IVSII-1G>A mutation and XmnI SNP from Thalassemia Research Center, Sari, Iran in 2015  were selected and categorized into two groups of 10 Responder (R) and 10 Non-Responder (NR) according to their clinical HU response. Ten healthy individuals as a control group were also selected. Hematopoietic erythroid progenitors were expanded from peripheral blood. Hb concentration was measured using photometry method. The flow cytometry and real-time PCR methods were applied for the analysis of cell surface markers (CD71 and CD235a) and gene expression (BCL11A and SOX6), respectively. Results: R and NR groups produced higher amount of Basic Hb than C group in cell culture medium at day 14 (P< 0.05). After HU treatment, in R group, Hb levels was significantly elevated in comparison to NR and C group (P< 0.05). BCL11A expression was decreased after exposure to HU in all groups while SOX6 expression was only down-regulated in C group, and its expression was increased in R and NR groups after HU treatment. Conclusion: Since different factors including wide networks of intracellular factors and individual differences between patients can affect response to HU in patients, the increasing Hemoglobin on culture medium alone cannot predict clinical responsiveness to that drug.    
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Mohammad Reza MAHDAVI
Dept. of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Farzin POURFARZAD
Dept. of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Mehrnoush KOSARYAN
Dept. of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Mohammad Taghi AKBARI
Dept. of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/10395
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/86/article-86-1354304.pdf
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات