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Iranian Journal of Biotechnology، جلد ۱۷، شماره ۱، صفحات ۷۴-۷۹
عنوان فارسی Expression, Purification, and Antiserum Production of the Truncated UL۳۱ Protein of Herpes Simplex Virus ۱
چکیده فارسی مقاله Background: The UL31 protein of herpes simplex virus 1 (HSV-1) plays an important role in the HSV-1 replication, however, its pinpoint functions in the life cycle of the virus have yet to be adequately elucidated.
Objectives: An antiserum specific for detecting HSV-1 UL31 was prepared as the foundation for future research on the role of UL31 in the course of HSV-1 infection.
Materials and Methods: Recombinant protein of UL31 was expressed in Escherichia coli, which was then purified and employed to raise the level of antiserum in mice. Subsequently, western blot and immunofluorescence assay (IFA) were utilized to detect the specific antiserum.
Results: The recombinant UL31 protein consisting of N-terminal 27 aa of UL31 was fused to EYFP and His-tag. It was expressed, purified, and applied to the preparation of the antiserum. Western blot analysis and IFA demonstrated that this antiserum could detect both the recombinant UL31 and the native UL31.
Conclusions: Our results manifest that this antiserum could be conducive to further investigations concerning the roles of UL31 in the HSV-1 infection.
کلیدواژه‌های فارسی مقاله Escherichia coli،، Herpes simplex virus 1،، Immune Sera،، Recombinant Proteins،
عنوان انگلیسی Expression, Purification, and Antiserum Production of the Truncated UL31 Protein of Herpes Simplex Virus 1
چکیده انگلیسی مقاله Background: The UL31 protein of herpes simplex virus 1 (HSV-1) plays an important role in the HSV-1 replication, however, its pinpoint functions in the life cycle of the virus have yet to be adequately elucidated.
Objectives: An antiserum specific for detecting HSV-1 UL31 was prepared as the foundation for future research on the role of UL31 in the course of HSV-1 infection.
Materials and Methods: Recombinant protein of UL31 was expressed in Escherichia coli, which was then purified and employed to raise the level of antiserum in mice. Subsequently, western blot and immunofluorescence assay (IFA) were utilized to detect the specific antiserum.
Results: The recombinant UL31 protein consisting of N-terminal 27 aa of UL31 was fused to EYFP and His-tag. It was expressed, purified, and applied to the preparation of the antiserum. Western blot analysis and IFA demonstrated that this antiserum could detect both the recombinant UL31 and the native UL31.
Conclusions: Our results manifest that this antiserum could be conducive to further investigations concerning the roles of UL31 in the HSV-1 infection.
کلیدواژه‌های انگلیسی مقاله Escherichia coli, Herpes simplex virus 1, Immune Sera, Recombinant Proteins
نویسندگان مقاله Xingmei Zou |
Department of Pathogenic Biology and Immunology, Sino-French Hoffmann Institute, School of Basic Medical Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou 511436, Guangdong, China

Zuo Xu |
Department of Pathogenic Biology and Immunology, Sino-French Hoffmann Institute, School of Basic Medical Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou 511436, Guangdong, China

Yuanfang Wang |
Department of Pathogenic Biology and Immunology, Sino-French Hoffmann Institute, School of Basic Medical Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou 511436, Guangdong, China

Ping Wang |
Guangzhou Medical University

Delong Liu |
GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou 511436, Guangdong, China

Ruiyi Luo |
GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou 511436, Guangdong, China

Yao Wang |
GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou 511436, Guangdong, China

Qiusan Chen |
The Third Clinical School of Guangzhou Medical University, No. 63 Duobao Road, Liwan District, Guangzhou 510150, Guangdong, China

Haifan Li |
School of Public Health, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou 511436, Guangdong, China

Hao Peng |
GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou 511436, Guangdong, China

Gengde Hong |
The Third Clinical School of Guangzhou Medical University, No. 63 Duobao Road, Liwan District, Guangzhou 510150, Guangdong, China

Jinyu Lin |
The Third Clinical School of Guangzhou Medical University, No. 63 Duobao Road, Liwan District, Guangzhou 510150, Guangdong, China

Meili Li |
Department of Pathogenic Biology and Immunology, Sino-French Hoffmann Institute, School of Basic Medical Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou 511436, Guangdong, China

Mingsheng Cai |
Department of Pathogenic Biology and Immunology, Sino-French Hoffmann Institute, School of Basic Medical Science, Guangzhou Medical University, Xinzao Town, Panyu, Guangzhou 511436, Guangdong, China

نشانی اینترنتی http://www.ijbiotech.com/article_85038_db7d63bfae737a49ba96148cc0283350.pdf
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