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Journal of Agricultural Science and Technology، جلد ۱۸، شماره ۲، صفحات ۴۲۳-۴۳۵
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عنوان انگلیسی Agrobacterium Mediated Transformation of Somatic Embryos of Persian Walnut Using fld Gene for Osmotic Stress Tolerance
چکیده انگلیسی مقاله Somatic embryos of Persian walnut were transformed with two strains of Agrobacterium tumefaciens i.e. LBA4404 and C58, and two plasmids, namely, pBI121 with nptII and gus genes for improving the transformation protocol, and p6u-ubi-FVTI plasmid containing the hpt and fld genes. The transformation frequency was 10%. PCR and RT-PCR analysis proved the presence and expression of the genes. The transgenic and non-transgenic somatic embryos of Persian walnut were exposed to four salinity levels (0, 50, 100, and 200 mM NaCl) and four osmotic stress (0, 1.5, 5, and 10% PEG) levels. After 20 days, the number of survived, secondary and cotyledonary somatic embryos, as well as fresh and dry weights of embryos were evaluated. In addition, the transgenic and non-transgenic regenerated plantlets with 3 leaves and 2.5 cm length were subjected to 200 mM NaCl. In both experiments, the main effects of fld-transformation and stress treatments on evaluated parameters were significant. Transgenic somatic embryos showed no significant differences at 0 and 200 mM NaCl and 0 and 1.5% PEG. Significant differences of transgenic vs. non-transgenic somatic embryos were observed at 50 and 100 mM NaCl and 5 and 10% PEG. Non-transgenic plantlets on medium containing 200 mM NaCl showed complete necrosis and died after 10 days, while transgenic lines continued growth until 45 days. Our results clearly showed that expression of fld gene increased stress tolerance in fld transformant lines of walnut, and also revealed that expression of this specific cyanobacterial protein may provide a powerful tool to improve tolerance to environmental stresses.
کلیدواژه‌های انگلیسی مقاله Flavodoxin,Salinity stress tolerance,Walnut transformation
نویسندگان مقاله محمدعلی شیخ بیگ گوهرریزی | m a sheikh beig goharrizi
department of horticulture, aburaihan campus, university of tehran, pakdasht, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

a دژآهنگ |
department of horticulture, aburaihan campus, university of tehran, pakdasht, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

m توحیدفر |
department of biotechnology, faculty of new technologies and energy engineering, shahid beheshti university, tehran, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

a ایزدی دربندی | izadi darbandi
department of agronomy and crops breeding sciences, aburaihan campus, university of tehran, pakdasht, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

c nestor |
institute of molecular and cell biology of rosario ibr-conicet - national university of rosario- rosario – argentina.

محمدرضا حاجی رضایی | m r
leibniz institute of plant genetics and crop plant research ipk , corrensstrasse 03, 06466 gatersleben, germany.

k وحدتی |
department of horticulture, aburaihan campus, university of tehran, pakdasht, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://jast.modares.ac.ir/article_14302_02e56a05b3eda85af8edb1acb2f6ad5e.pdf
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