این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Agricultural Science and Technology، جلد ۱۷، شماره ۵، صفحات ۱۳۰۳-۱۳۱۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Identification of Possible Mechanisms of Chickpea (Cicer arietinum L.) Drought Tolerance Using cDNA-AFLP
چکیده انگلیسی مقاله Drought sensitivity is considered as a major concern for chickpea (C. arietinum) seed production. Determination of drought adaptation mechanisms is an essential constituent of this crop breeding programs. With this purpose, the present research was conducted to distinguish the molecular basis of chickpea drought tolerance using cDNA-AFLP approach. The expression profile was compared between drought tolerant (ICCV2 and FLIP9855C) and susceptible lines (ILC3279) of chickpea under three drought treatments including well-watered, intermediate, and severe stress; where soil water content was kept at 85–90%, 55–60%, and 25–30% of Field capacity, respectively. Totally, 295 transcript-derived fragments (TDFs) were visualized. Among the differentially expressed TDFs, 72 TDFs were sequenced. Sequenced cDNAs were categorized in different functional groups involved in macromolecules metabolism, cellular transport, signal transduction, transcriptional regulation, cell division and energy production. Based on the results, ribosomal protein S8, mitochondrial chaperone, proteases, hydrolases, UDP -glucuronic acid decarboylase, 2-hydroxyisoflavanone dehydratase, NADPH dehydrogenase, chloride channels, calmodulin, ABC transporter, histone deacetylase and factors involved in chloroplast division were among genes that were affected by drought stress. Similarity search in data base showed that cell wall elasticity, isoflavonoids, maintenance of structure and function of proteins through increase in expression of mitochondrial chaperones, programed cell death, and remobilization of storage material from leaves to seeds were among mechanisms that distinguished differences between drought tolerant and drought susceptible lines.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله m پوراسماعیل |
department of plant physiology, faculty of biological sciences, kharazmi university, 15719-14911, tehran, islamic republic of iran.

j مظفری |
national plant gene bank, seed and plant improvement institute, mahdasht avenue, karaj, islamic republic of iran.

رضاعلی خاوری نژاد | r a khavari nejad
department of plant physiology, faculty of biological sciences, kharazmi university, 15719-14911, tehran, islamic republic of iran.

f نجفی |
department of plant physiology, faculty of biological sciences, kharazmi university, 15719-14911, tehran, islamic republic of iran.

f مرادی |
department of molecular physiology, agriculture biotechnology research institute, karaj, islamic republic of iran.

نشانی اینترنتی http://jast.modares.ac.ir/article_13259_8816b2fc45e0c1b0c2330f3e5774cd85.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات