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Journal of Agricultural Science and Technology، جلد ۱۵، شماره ۲، صفحات ۳۶۹-۳۷۹
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عنوان انگلیسی Coordinate Up‏-‏regulation of Vacuolar Pyrophosphatase and V-Na+/H+ ‎Antiporter to Early Salt Stress in Halophytic Monocot ‎Leptochloa fusca Roots ‎
چکیده انگلیسی مقاله Vacuolar H+-translocation pyrophosphatase (V-PPase) and Na+/H+ antiporter (V-Na+/H+) are important transporters in plant cells and have essential roles against abiotic stresses. In this study, the effects of salt stress were surveyed on the transcription of V-PPase pump and Na+/H+ antiporter genes at early times of NaCl treatment in roots of halophytic grass Leptochloa fusca through the Real Time PCR. The first results showed that L. fusca has high ability for accumulating Na+ under saline conditions. Real-Time PCR analysis demonstrated that the expression level of V-PPase and V-Na+/H+ increased in response to different levels of NaCl treatment, but the transcriptional increase of V-Na+/H+ gene was higher than that of the V-PPase gene, indicating that V-Na+/H+ had a crucial role in salt tolerance in this plant. These results indicated that coordinate up-regulations of V-PPase expression and Na+/H+ antiporter expression were correlated with Na+ sequestering into vacuoles of L. fusca. It was also shown that increase e in the expression level of V-Na+/H+ and V-PPase was correlated with the accumulation of sodium in roots, suggesting a physiological role for these antiporters and pump in Na+ compartmentation during adaptation to high salinity.
کلیدواژه‌های انگلیسی مقاله Na+ accumulation,Real time PCR,Transcript.‎
نویسندگان مقاله f شهریاری احمدی | shahriari ahmadi
department of biotechnology and plant breeding, ferdowsi university of mashhad, mashhad, islamic ‎republic of iran.‎
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

b پناهی |
department of biotechnology and plant breeding, ferdowsi university of mashhad, mashhad, islamic ‎republic of iran.‎
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

h مرعشی |
department of biotechnology and plant breeding, ferdowsi university of mashhad, mashhad, islamic ‎republic of iran.‎
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

n مشتاقی |
department of biotechnology and plant breeding, ferdowsi university of mashhad, mashhad, islamic ‎republic of iran.‎
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

a میر شمسی کاخکی | mirshamsi kakhki
department of biotechnology and plant breeding, ferdowsi university of mashhad, mashhad, islamic ‎republic of iran.‎
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

نشانی اینترنتی http://jast.modares.ac.ir/article_4955_f0c868ab2571d4b62dfb3eba942a6f9b.pdf
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