این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Caspian Journal of Enviromental Sciences، جلد ۱۷، شماره ۱، صفحات ۱-۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Extraction, purification and characterization of peroxidase from Vitis vinifera wastes
چکیده انگلیسی مقاله The proper consumption of plant wastes could not only provide a possible source of natural products, but it also is an environmental friendly research. The aim of this study was to use grape wastes as a source of peroxidase.  In practice, one isoenzyme of peroxidase (POD1) was partially purified from Vitis vinifera wastes, the plant which is widely harvested in Iran. The activity of this novel peroxidase was determined using guaiacol as its substrate. The new peroxidase was partially purified and its kinetic parameters determined. The values of Km and Vmax of peroxidase for guaiacol were 83.2 mM and 0.35 M/min respectively. Optimum pH and temperature were determined for guaiacol to be 6.2 and 60°C respectively. According to SDS-PAGE results, the molecular weight of isozymes was 38-40 KD. The results indicate that agricultural leftovers from Vitis vinifera are a considerable source for a peroxidase with reliable kinetic behaviors.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله K Alijanipoor |
Department of Biology, Faculty of Sciences, Payame Noor University, Tehran, Iran

R Hajihosseini |
Department of Biology, Faculty of Sciences, Payame Noor University, Tehran, Iran

R Sariri |
Department of Biology, University of Guilan, Rasht, Iran

A Vaziri |
Department of Biology, Faculty of Sciences, Payame Noor University, Tehran, Iran

نشانی اینترنتی https://cjes.guilan.ac.ir/article_3339_ce69d560098296ffcf592622eaa8f47b.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/385/article-385-1383084.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات