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Journal of Crop Protection، جلد ۴، شماره ۴، صفحات ۵۳۳-۵۴۳
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عنوان انگلیسی Elimination of Bean yellow mosaic virus through thermotherapy combined with meristem-tip culture in gladiolus corms
چکیده انگلیسی مقاله Bean yellow mosaic virus (BYMV, Potyvirus, Potyviridae) causes serious disease in Gladiolus spp. In this work, the possibility of obtaining BYMV free plant material from virus infected gladiolus corms was studied. Thermotherapy, meristem-tip culture and combination of both techniques on infected corms/meristem-tip explants (0.5–1 mm in length) resulted in BYMV elimination up to 15.38, 78.04 and 86.66%, respectively, as determined by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) and reverse transcription-polymerase chain reaction (IC-RT-PCR). Individual virus-free shoots readily rooted in vitro and were transferred to corm formation medium. The results showed that thermotherapy promotes the survival rate of explants during meristem-tip culture steps (except regeneration step) and also plantlet acclimatization. Statistical analysis showed that the BYMV elimination in gladiolus corms was significantly (P ≤ 0.01) affected by thermotherapy treatment of infected corms. Thermotherapy combined with meristem culture can greatly improve BYMV elimination efficiency from infected gladiolus corms, resulting in the production of BYMV free gladiolus plants.
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نویسندگان مقاله پریسا شریفی nezamabad | sharifi nezamabad
department of plant protection, faculty of agricultural sciences and engineering, university of tehran, karaj, iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

مینا کوهی حبیبی | koohi habibi
department of plant protection, faculty of agricultural sciences and engineering, university of tehran, karaj, iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

اکبر دیزجی |
department of plant protection, faculty of agricultural sciences and engineering, university of tehran, karaj, iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

سیامک کلانتری |
department of horticulture science, faculty of agricultural sciences and engineering, university of tehran, karaj, iran.
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://jcp.modares.ac.ir/article_13388_2ad448710f9bc6ed612b4251677e77a2.pdf
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