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Journal of Crop Protection، جلد ۳، شماره ۲، صفحات ۲۴۵-۲۵۴
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عنوان انگلیسی Characterization of digestive α-amylase in the midgut of willow leaf beetle Plagiodera versicolora (Coleoptera: Chrysomelidae)
چکیده انگلیسی مقاله Willow leaf beetle, Plagiodera versicolora is an important pest of willow trees that feeds on the leaves both as larvae and as adults. -Amylases (EC 3.2.1.1) are the major insect digestive enzymes that catalyze the endohydrolysis of long -1,4-glucan chains such as starch and glycogen. In the current study, -amylase activity was studied in the midgut of larvae and adults of P. versicolora. Amylase activity in the midgut of larvae and adults was 0.6807 and 0.1162 µmol/min/mg protein, respectively. The optimal pH for -amylase activity of larvae and adults was 4 and 8, respectively and the optimal temperature for both was 35 °C. The enzyme activity of larvae was inhibited by the addition of Na2+, K+ and Zn2+. K+ (at 5 mmol) had the most positive effect on α-amylase activity in adults. EGTA had significant influence on decreasing the enzyme activity in larvae. EDTA had the most effect on increasing the activity of the enzyme in adults. Plant amylase inhibitors play important role against insect pests. Therefore, the characterization of digestive enzymes and the examination of inhibitors on the enzyme activity could be useful in tackling insect pests.
کلیدواژه‌های انگلیسی مقاله Plagiodera versicolora,midgut,α- amylase
نویسندگان مقاله meysareh shabarari |
department of plant protection, faculty of agricultural sciences, university of mohaghegh ardabili, 56199-11367, ardabil, iran.

بهرام ناصری |
department of plant protection, faculty of agricultural sciences, university of mohaghegh ardabili, 56199-11367, ardabil, iran.

آرش زیبایی |
department of plant protection, faculty of agricultural sciences, university of guilan, 41635-1314, rasht, iran.
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)

جلیل حاجی زاده |
department of plant protection, faculty of agricultural sciences, university of guilan, 41635-1314, rasht, iran.
سازمان اصلی تایید شده: دانشگاه گیلان (Guilan university)

نشانی اینترنتی http://jcp.modares.ac.ir/article_10439_4f38f36f79787a6caf9bc2002795aac9.pdf
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