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Journal of Crop Protection، جلد ۱، شماره ۴، صفحات ۳۳۷-۳۴۷

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عنوان انگلیسی Transcript analysis of some pathogenicity-related elements in an Iranian A* isolate of Xanthomonas citri subsp. citri
چکیده انگلیسی مقاله Asiatic citrus canker is a devastating disease resulting in drastic economic losses in citriculture worldwide. Amongst three different types of the disease, i.e. A, A* and Aw, the A* type is genetically less known. In order to comprehend the behavior of the Asiatic citrus canker A*-type strain (Xanthomonas citri subsp. citri) in the vicinity of the host cells, a targeted semi-quantitative transcript analysis approach via RT-PCR was carried out. A subset of sixteen genes, as representative of different steps involved in phytopathogencity, was analyzed on the culture medium (as uninduced) and compared with the subset isolated from the infected Mexican lime (Citrus auarntifolia L.) plants (as induced). The results showed that certain genes were up-regulated in induced condition, suggesting a putative role in bacteria-host interaction. Furthermore, the transcripts in induced condition could be classified into constitutive, early- and late-responsive genes, demonstrating their functional relevance during the host-pathogen interaction.
کلیدواژه‌های انگلیسی مقاله RT-PCR,Xcc,citrus canker,Mexican lime

نویسندگان مقاله رعنا روحانی |
department of plant biotechnology, national institute for genetic engineering and biotechnology nigeb , p. o. box 14155-6343, tehran, iran

مسعود سلطانی نجف آبادی | soltani najafabadi
oil seeds research department, seed and plant improvement institute spii , karaj, iran

سید مهدی علوی | seyed mehdi
dept. of plant biotechnology, national institute for genetic engineering and biotechnology nigeb

ناصر فرخی |
department of agronomy and plant breeding, faculty of agricultural engineering, shahrood university of technology, p. o. box 361999516, shahrood, iran
سازمان اصلی تایید شده: دانشگاه صنعتی شاهرود (Shahrood university of technology)

مسعود شمس بخش | shams bakhsh
plant pathology dept., faculty of agriculture, tarbiat modares university t. m. u. , tehran-iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)


نشانی اینترنتی http://jcp.modares.ac.ir/article_364_676165790111f2a0abdb95c638d0dbb7.pdf
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