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Journal of Livestock Science and Technology، جلد ۴، شماره ۱، صفحات ۷۱-۷۶
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عنوان انگلیسی Single nucleotide polymorphism of the lactoferrin gene and its association with milk production and reproduction traits in Iranian Holstein cattle
چکیده انگلیسی مقاله Bovine lactoferrin (LTF) is a member of the transferrin family of iron-binding proteins. This protein is present in a wide variety of biological fluids and shows important physiological functions in body. In this study, 404 blood samples were collected from Holstein dairy farms in Iran. A 301 bp fragment of intron 6 in bovine LTF gene was amplified and the animals were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Two alleles of bovine LTF, A and B, were identified in the studied population. The frequency of the A and B (mutant type) alleles were estimated to be 0.803 and 0.197, respectively. The alleles controlled the occurrence of two genotypes, AA and AB, with frequency of 0.606 and 0.393, respectively. GLM (Generalized linear Model) analysis was applied to evaluate the association of bovine LTF with milk production (305-day milk yield, fat and protein percentage) and reproductive traits (pregnancy length (d), milking days (d) and somatic cell score, SCS). It was found that AB cows showed significantly higher (P < 0.05) fat percentage and SCS in milk in comparison with AA genotype. Other traits did not show any significant difference. Regarding the association revealed, the SNP has the potential to be considered as a marker in marker-assisted selection.
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نویسندگان مقاله h اسداله پور nanaei | asadollahpour nanaei
department of animal sciences, college of agriculture, isfahan university of technology, isfahan 84156-83111, iran. | department of animal science, faculty of agriculture, shahid bahonar university of kerman, kerman, iran.
سازمان اصلی تایید شده: دانشگاه صنعتی اصفهان (Isfahan university of technology)

s انصاری مهیاری | ansari mahyari
department of animal sciences, college of agriculture, isfahan university of technology, isfahan 84156-83111, iran.
سازمان اصلی تایید شده: دانشگاه صنعتی اصفهان (Isfahan university of technology)

محمدعلی ادریس | m a
department of animal sciences, college of agriculture, isfahan university of technology, isfahan 84156-83111, iran.
سازمان اصلی تایید شده: دانشگاه صنعتی اصفهان (Isfahan university of technology)

نشانی اینترنتی http://lst.uk.ac.ir/article_1384_218.html
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