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Journal of Livestock Science and Technology، جلد ۱، شماره ۱، صفحات ۲۹-۳۴
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عنوان انگلیسی Association of TG-repeats in the 5’-flanking region of bovine growth hormone receptor (GHR) gene with milk production traits and somatic cell count in Holstein cattle
چکیده انگلیسی مقاله The growth hormone receptor (GHR) is a member of cytokine/hematopoietin family that mediates the biological actions of growth hormone (GH) on target tissues. Therefore, the purpose of this study was to examine the association of TG-repeat polymorphisms in the 5’-flanking region of bovine GHR gene with milk production traits and somatic cell score (SCS) in Holstein cattle of Iran. The part of 5’-flanking region of GHR gene that encompassed TG-repeat was screened by single strand conformation polymorphism (SSCP) method and DNA sequencing. Five hundred eighteen Iranian Holstein cows were genotyped, giving 3 distinct SSCP patterns (A, B, and C). Frequencies of these patterns for the amplified fragment were 0.21, 0.26 and 0.53, respectively. Statistical analysis revealed that TG-repeat had a significant effect on average daily milk production (P < 0.05) and tended to associate with fat and protein percentage. No significant difference was observed between TG-genotypes and SCS. The association identified in the TG-repeat of GHR gene may have potential to serve as candidate genetic marker for marker assisted selection (MAS) in cattle.
کلیدواژه‌های انگلیسی مقاله GHR gene, 5’-flanking region, SSCP, microsatellite, Cattle
نویسندگان مقاله m محقق دولت آبادی دولت آبادی | muhaghegh dolatabady
department of animal science, faculty of agriculture, yasouj university, yasouj, iran
سازمان اصلی تایید شده: دانشگاه یاسوج (Yasouj university)

j حبیبی زاد |
department of animal science, faculty of agriculture, yasouj university, yasouj, iran
سازمان اصلی تایید شده: دانشگاه یاسوج (Yasouj university)

محمدرضا بحرینی بهزادی | m r bahreini behzadi
department of animal science, faculty of agriculture, yasouj university, yasouj, iran
سازمان اصلی تایید شده: دانشگاه یاسوج (Yasouj university)

نشانی اینترنتی http://lst.uk.ac.ir/article_481_70.html
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