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فرآیند و کارکرد گیاهی، جلد ۷، شماره ۲۸، صفحات ۵۱-۶۰
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چکیده فارسی مقاله
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عنوان انگلیسی Tissue Culture and Production of Volatile Secondary Metabolites from Callus of Stevia rebaudiana at Different Combination of Plant Growth Regulators
چکیده انگلیسی مقاله  Stevia rebaudiana Bert (Asteraceae) is herbaceous plant with sweet leaves, which are the source of sweetener products. The present study focuses on effects of different combination of plant growth regulators on callus induction, callus cell growth and regeneration and volatile constituents from callus of stevia in tissue culture condition. The results of present study showed callus formation promoted in all of media contained phytohormones of different auxin/cytokinin based combinations. However, NAA/BA combinations are significantly more effective to callus initiation and the highest amount of callus was obtained in medium supplemented with 1.5:2 mg/L of NAA/BA. It was revealed that shoot induction in callus was happen at hormonal treatments as IBA/BA and IBA/KIN (0.5:1.5 mgl-1). High rooting obtained in media supplemented with NAA/KIN (1.5:0.5 mgl-1). The results of GC-MS analyses of volatile constituents of calli showed that the callus oil obtained in media supplemented with NAA/Kin (1.5:0.5 mgl-1). The NAA/Kin treatment with 1:0.5 mgl-1 concentration was dominated with squalene (40.50%) as main compound. The callus formed in media contained NAA/BA was enriched with Tricosane (38.57%). In addition, phytol (11.69%, 6.92%) was regarded as major constituent of the oil obtained from cultures supplemented with IBA/Kin and IBA/BA, respectively. It was concluded that there was a considerable differences in the essential oil profile of callus induced from different hormonal combinations. It is thought that various hormonal treatments may be tending to changes in metabolic pathways.  
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نویسندگان مقاله | Seyed Mehdi Razavi
University of Mohaghegh Ardabili


| Farahrouz Zahri
University of Mohaghegh Ardabili


| Mesomeh Abedini
University of Mohaghegh Ardabili
نشانی اینترنتی http://jispp.iut.ac.ir/browse.php?a_code=A-10-621-8&slc_lang=en&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/999/article-999-1393249.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده تنظیم کننده های رشد گیاهی
نوع مقاله منتشر شده پژوهشی
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