| چکیده انگلیسی مقاله |
Prepulse Inhibition (PPI), the objective measure of sensorimotor gating disturbance has being widely used in animal models of schizophrenia. Dopaminergic direct and indirect agonists impair PPI. However, the profile of dopaminergic receptors involved in PPI impairment by dopamine agonists is not clear. By injecting shRNA expressing plasmids against dopamine D2 receptor genes (DRD2) in the nucleus accumbens, here, we studied the effect of apomorphine on PPI in D2 down-regulated rats. Seventy two adult Wistar rats assigned randomly in nine groups, each received coding (250 and 500 ng/µl) or noncoding shRNA expressing plasmids against DRD2 in the nucleus accumbens, with or without apomorphine (0.5 mg/kg, S.C., 72 hours after treatment with plasmids). Auditory startle response and PPI were measured after apomorphine injection. Real time RT-PCR was used to measure DRD2 expression. Results showed that apomorphine significantly decreased PPI in noncoding plasmid treated rats; While, PPI did not impaired in rats pretreated with 250 and 500 ng/µl shRNA expressing plasmids. Accordingly, the expression of DRD2 mRNA in the nucleus accumbens showed 72-78% decrease in expressing plasmid treated rats. Additionally, treatment with expressing plasmids had no effect on basal PPI and/or auditory startle response. Taken together, our results demonstrated that DRD2 silencing in the nucleus accumbens can prevent PPI impairment by apomorphine. These observations suggest application of molecular techniques such as the use of shRNA against DRD2s in studies of schizophrenia pathophysiology and development of new treatments in schizophrenia. |
| نویسندگان مقاله |
محمدرضا نوری دلویی | m r noori daloii
department of medical genetics, faculty of medicine, tehran university of medical sciences, tehran, islamic republic of iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
a شهبازی |
physiology research center, iran university of medical sciences, tehran, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
s علیزاده zendehrood | alizadeh zendehrood
physiology research center, iran university of medical sciences, tehran, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
a شایان نیا | shayan nia
department of medical genetics, faculty of medicine, tehran university of medical sciences, tehran, islamic republic of iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
m مجرد |
department of medical genetics, faculty of medicine, tehran university of medical sciences, tehran, islamic republic of iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
m خیرالهی |
department of medical genetics, faculty of medicine, tehran university of medical sciences, tehran, islamic republic of iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
a رشیدی نژاد | rashidi nezhad
department of medical genetics, faculty of medicine, tehran university of medical sciences, tehran, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
a حق پرست |
neuroscience research center, shahidbeheshti university of medical sciences, tehran, islamic republic of iran.
f زارع مهرجردی | zare mehrjerdi
physiology research center, iran university of medical sciences, tehran, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
s نیک نظر |
physiology research center, iran university of medical sciences, tehran, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
m مستوفی |
physiology research center, iran university of medical sciences, tehran, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
h هاشمی sotoobadi | hashemi sotoobadi
physiology research center, iran university of medical sciences, tehran, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
m خاکساری |
physiology department, shahrood university of medical sciences, shahrood, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شاهرود (Shahrood university of medical sciences)
علیرضا نوری دلویی | a r noori daloii
department of medical genetics, faculty of medicine, tehran university of medical sciences, tehran, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
a محمدی |
neuroscience research center, baqiyatallah university of medical sciences, tehran, islamic republic of iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)
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