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Molecular Biology Research Communications، جلد ۵، شماره ۳، صفحات ۱۶۷-۱۷۴
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عنوان انگلیسی Microsatellite (SSR) amplification by PCR usually led to polymorphic bands: Evidence which shows replication slippage occurs in extend or nascent DNA strands
چکیده انگلیسی مقاله Microsatellites or simple sequence repeats (SSRs) are very effective molecular markers in population genetics, genome mapping, taxonomic study and other large-scale studies. Variation in number of tandem repeats within microsatellite refers to simple sequence length polymorphism (SSLP); but there are a few studies that are showed SSRs replication slippage may be occurred during in vitro amplification which are produced ‘stutter products’ differing in length from the main products. The purpose of this study is introducing a reliable method to realize SSRs replication slippage. At first, three unique primers designed to amplify SSRs loci in the great gerbil (Rhombomys opimus) by PCR. Crush and soak method used to isolate interesting DNA bands from polyacrylamide gel. PCR products analyzed using by sequencing methods. Our study has been shown that Taq DNA polymerase slipped during microsatellite in vitro amplification which led to insertion or deletion of repeats in sense or antisense DNA strands. It is produced amplified fragments with various lengths in gel electrophoresis showed as ‘stutter bands’. Thus, in population studies by SSRs markers recommend that replication slippage effects and stutter bands have been considered.
کلیدواژه‌های انگلیسی مقاله Microsatellites,Taq polymerase slippage,Replication slippage
نویسندگان مقاله اباصلت حسین زاده کلاگر | hossienzadeh colagar
department of molecular and cell biology, faculty of basic sciences, university of mazandaran, babolsar, mazandaran, iran
سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)

محمد جواد حقیقت نیا | mohammad javad
department of molecular and cell biology, faculty of basic sciences, university of mazandaran, babolsar, mazandaran, iran
سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)

زهرا امیری |
department of molecular and cell biology, faculty of basic sciences, university of mazandaran, babolsar, mazandaran, iran
سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)

مریم مهاجرانی |
department of molecular and cell biology, faculty of basic sciences, university of mazandaran, babolsar, mazandaran, iran
سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)

مجید تفریحی |
department of molecular and cell biology, faculty of basic sciences, university of mazandaran, babolsar, mazandaran, iran
سازمان اصلی تایید شده: دانشگاه مازندران (Mazandaran university)

نشانی اینترنتی http://mbrc.shirazu.ac.ir/article_3789_10b7d720b5b93b199c8c2fc912c351fc.pdf
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