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Molecular Biology Research Communications، جلد ۴، شماره ۱، صفحات ۴۳-۵۵
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عنوان انگلیسی Characterization of Iranian Nonaflatoxigenic Strains of Aspergillus flavus Based on Microsatellite-primed PCR
چکیده انگلیسی مقاله Out of fifty-two Iranian nonaflatoxigenic strains of Aspergillus flavus, which were collected from various substrates (soil and kernel) and sources (peanut, corn and pistachio), fifteen representatives were selected according to their different geographical origins (six provinces: Guilan and Golestan, Ardebil, Fars, Kerman and Semnan) and vegetative compatibility groups (VCGs, IR1 to IR15) for microsatellite-primed PCR analysis. Two inter-simple sequence repeat (ISSR) primers AFMPP and AFM13 were used to determine the polymorphism and the relationship among strain isolates. The A. flavus isolates were identified by their morphologies and their identities were confirmed by PCR amplification using the specific primer pair ITS1 and ITS4. The results revealed variations in the percentages of polymorphisms. In ISSR analysis, primers AFMPP and AFM13 generated a total of 18 and 23 amplicons among the fungal strains, which 12 (66.7%) and 22 (95.7%) were polymorphic, respectively. Cluster analysis of ISSR data was carried out by using 1 D DNA gel image analysis. The two dendrograms obtained through these markers showed six different clustering of testing nonaflatoxigenic A. flavus L strains, but we noted that some clusters were different in some cases. The microsatellite-primed PCR data revealed that the Iranian nonaflatoxigenic isolates of A. flavus were not clustered based on their origins and sources. This study is the first to characterize Iranian nonaflatoxigenic isolates of A. flavus using ISSR markers.
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نویسندگان مقاله محمود هوشیار فرد |
plant protection department faculty of agriculture ferdowsi university mashhad iran
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

حمید روحانی |
department of plant protection, faculty of agriculture, ferdowsi university, mashhad
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

ماهرخ فلاحتی رستگار | falahati rastegar
department of plant protection, faculty of agriculture, ferdowsi university, mashhad
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

سعید ملک زاده شفارودی | malekzadeh shafaroudi
department of agricultural biotechnology, faculty of agriculture, ferdowsi university, mashhad, iran
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

عصمت مهدیخانی مقدم | mahdikhani moghaddam
department of plant protection faculty of agriculture ferdowsi university
سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

نشانی اینترنتی http://mbrc.shirazu.ac.ir/article_2908_6dedee9e6bc85d3ae69aed1e7d93c2b9.pdf
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