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Molecular Biology Research Communications، جلد ۲، شماره ۴، صفحات ۱۴۳-۱۴۹
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عنوان انگلیسی Phylogenetic analysis of Escherichia coli strains isolated from human samples
چکیده انگلیسی مقاله Escherichia coli (E. coli) is a normal inhabitant of the gastrointestinal tract of vertebrates, including humans. Phylogenetic analysis has shown that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D). Group A and B1 are generally associated with commensals, whereas group B2 is associated with extra-intestinal pathotypes. Most enteropathogenic isolates, however, are assigned to group D. In the present study, a total of 102 E. coli strains, isolated from human samples, were used. Phylogenetic grouping was done based on the Clermont triplex PCR method using primers targeted at three genetic markers, chuA, yjaA and TspE4.C2. Group A contained the majority of the collected isolates (69 isolates, 67.64%), followed by group B2 (18 isolates, 17.64%) and D (15 isolates, 14.7%) and no strains were found to belong to group B1. The distribution of phylogenetic groups in our study suggests that although the majority of strains were commensals, the prevalence of enteropathogenic and extra-intestinal pathotypes was noteworthy. Therefore, the role of E. coli in human infections including diarrhea, urinary tract infections and meningitis should be considered.
کلیدواژه‌های انگلیسی مقاله Escherichia coli,Phylogenetic grouping,Triplex PCR,Human
نویسندگان مقاله عبدالله درخشنده |
department of pathobiology school of veterinary medicine shiraz university shiraz, 71345-1731 iran
سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

رویا فیروزی |
department of pathobiology school of veterinary medicine shiraz university shiraz, 71345-1731 iran
سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

محمد معتمدی فر |
dept. of bacteriology amp;amp; virology shiraz medical school shiraz university of medical sciences p.o. box 71455-119, shiraz 71455, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شیراز (Shiraz university of medical sciences)

آذر معتمدی |
phd student in department of pathobiology school of veterinary medicine shiraz university shiraz, 71345-1731 iran
سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

مریم بهادری |
phd student in department of pathobiology school of veterinary medicine shiraz university shiraz, 71345-1731 iran
سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

زهرا نظیری |
phd student in department of pathobiology school of veterinary medicine shiraz university shiraz, 71345-1731 iran
سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

نشانی اینترنتی http://mbrc.shirazu.ac.ir/article_1822_119bce9ed57f4bf4c888d20856596514.pdf
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