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Molecular Biology Research Communications، جلد ۱، شماره ۱، صفحات ۲۷-۳۲
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عنوان انگلیسی Inhibition of chickpea seedling copper amine oxidases by tetraethylenepentamine
چکیده انگلیسی مقاله Copper amine oxidases are important enzymes, which contribute to the regulation of mono- and polyamine levels. Each monomer contains one Cu(II) ion and 2,4,5-trihydroxyphenylalanine (TPQ) as cofactors. They catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. The mechanism is followed by the transfer of two electrons to molecular oxygen which is reduced to hydrogen peroxide. Inhibitors are important tools in the study of catalytic properties of copper amine oxidases and they also have a wide application in physiological research. In this study, purification of the chickpea seedling amine oxidase, was done via salting out by ammonium sulfate and dialysis, followed by DEAE-cellulose column chromatography. By using the Lineweaver - Burk plot, the Km and Vm of the enzyme were found to be 3.3 mM and 0.95 mmol/min/mg, respectively. In this study, the interaction of chickpea diamino oxidase with tetraethylene- pentamine was studied. Analysis of kinetic data indicated that tetraethylenepentamine (with Ki=0.1 mM) inhibits the enzyme by linear mixed inhibitory effect.
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نویسندگان مقاله سونا طلایی |
deptarment of biology, faculty of science, university of mohaghegh ardabili, ardabil, iran

اسدالله اسدی |
deptarment of biology, faculty of science, university of mohaghegh ardabili, ardabil, iran

مجتبی امانی |
deptarment of science, faculty of medicine, university of medical science, ardabil, iran

نشانی اینترنتی http://mbrc.shirazu.ac.ir/article_238_5a2a3e5d850bcf7d77885214c6a69bdf.pdf
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