این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Veterinary Research Forum، جلد ۷، شماره ۳، صفحات ۱۸۱-۱۸۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Expression of pluripotent stem cell markers in mouse uterine tissue during estrous cycle
چکیده انگلیسی مقاله It was assumed that uterine stem cells are responsible for the unique regenerative capacity of uterine. Therefore, the aim of the present study was to investigate the expression of the pluripotent stem cell markers in the mice uterine tissue during different stages of estrous cycles. Twelve virgin female NMRI mice (6 to 8 weeks old) were considered at proestrus, estrus, metestrus and diestrus according to the cell types observed in the vaginal smear and underwent hysterectomy operation. Quantitative real-time polymerase chain reaction (PCR) and immunohistochemical staining for pluripotent stem cell markers (SOX2, OCT4, KLF4, and NANOG) were performed. Immunofluorescence staining revealed that expression and localization of the pluripotency markers SOX2, OCT4, KLF4, and NANOG at the protein level were not different throughout estrous cycle. Also, mRNA of pluripotency markers was detected in all tested samples. However, there were no significant differences in their genes expression at each stage and during the estrous cycle. Different hormonal profile during the estrous cycle could not affect expression of pluripotent stem cell markers in uterine tissue.
کلیدواژه‌های انگلیسی مقاله Estrous Cycle,Mice,Pluripotent Stem Cells,Uterine Tissue
نویسندگان مقاله کلثوم چوبینه |
department of cellular and molecular biology, school of biology, damghan university, damghan, iran
سازمان اصلی تایید شده: دانشگاه دامغان (Damghan university)

سعید زواره |
department of cellular and molecular biology, school of biology, damghan university, damghan, iran
سازمان اصلی تایید شده: دانشگاه دامغان (Damghan university)

مژده صالح نیا |
department of anatomy, faculty of medical sciences, tarbiat modares university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

محمد تقی قربانیان | mohamad taghi
department of cellular and molecular biology, school of biology, damghan university, damghan, iran
سازمان اصلی تایید شده: دانشگاه دامغان (Damghan university)

سید حسن پای لاخی | seyed hassan
department of cellular and molecular biology, school of biology, damghan university, damghan, iran
سازمان اصلی تایید شده: دانشگاه دامغان (Damghan university)

نشانی اینترنتی http://vrf.iranjournals.ir/article_21611_caf650095206af1a95e34bcbbb990c8c.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات