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Veterinary Research Forum، جلد ۴، شماره ۱، صفحات ۳۷-۴۱
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عنوان انگلیسی Follicle stimulating hormone increases spermatogonial stem cell colonization during in vitro co-culture
چکیده انگلیسی مقاله The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. To investigate and biomanipulation of these cells, proliferation and viability rate of cells should be increased in vitro, at first. Follicle stimulating hormone (FSH) has been suggested to play a determinant role in the survival of germ cells in addition to increasing spermatogonial proliferation. In this study, the in vitro effects of FSH on spermatogonial cell colony formation were investigated. Sertoli and spermatogonial cells were isolated from 3-5 months old calves. The identity of the Sertoli cells and spermatogonial stem cells were confirmed through immunocytochemistry and colony morphology, respectively. Co-cultured Sertoli and spermatogonial cells were treated with FSH in different dose of 10, 20 and 40 IU mL-1 FSH, before colony assay. Results indicated that, FSH increased in vitro colonization of spermatogonial cells in comparison with control group. In conclusion, using FSH provided proper bovine spermatogonial stem cell culture medium for in vitro study of these cells.
کلیدواژه‌های انگلیسی مقاله Bovine,Co-culture,FSH,Sertoli,SSCs
نویسندگان مقاله رضا نارنجی ثانی | narenji sani
department of theriogenology, faculty of veterinary medicine, university of tehran, tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

پرویز تاجیک |
department of theriogenology, faculty of veterinary medicine, university of tehran, tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

محمد حسن یوسفی | mohammad hassan
faculty of veterinary medicine, semnan university, semnan, iran
سازمان اصلی تایید شده: دانشگاه سمنان (Semnan university)

منصوره موحدین |
department of anatomical sciences, school of medical sciences, tarbiat modarres university, tehran, iran
سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

بابک قاسمی پناهی | qasemi panahi
department of animal science, faculty of agriculture, university of tabriz, tabriz, iran
سازمان اصلی تایید شده: دانشگاه تبریز (Tabriz university)

شیوا شفیعی |
department of theriogenology, faculty of veterinary medicine, university of tehran, tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

محمود احمدی همدانی | ahmadi hamedani
faculty of veterinary medicine, semnan university, semnan, iran
سازمان اصلی تایید شده: دانشگاه سمنان (Semnan university)

نشانی اینترنتی http://vrf.iranjournals.ir/article_1957_1fa4b82b946d0d5b0d7c882722d78ec0.pdf
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