این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۲۳، شماره ۴، صفحات ۲۹۷-۳۰۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The Inhibitory Effect of Mouse Gastric DNA on Amplification of Helicobacter pylori Genomic DNA in Quantitative PCR
چکیده انگلیسی مقاله Background: Quantitation of Helicobacter pylori (Hp) in the gastric tissue is essential for assessment of vaccination/therapeutic regimens. Methods & Results: Here, the inhibitory effect of mouse gastric DNA (MgDNA) on amplification of Hp genomic DNA (HpDNA) was evaluated by spiking HpDNA with serial dilutions of MgDNA, which yielded concentrations of >10 ng/µl and >0.63-10 ng/µl of MgDNA, as inhibition and interference zones, respectively. Mice were then inoculated with varying doses of Hp and assessed at the inhibition-free concentration of 0.63 ng/µl. The average Hp copy numbers per microgram of gastric tissue discriminated mice having received high vs. low dose inoculums (p < 0.001). Secondly, Hp copy numbers were quantitated in immunized mice, which demonstrated significantly lower numbers, in reference to controls (p = 0.006). Conclusion: Our method, bypassing the inhibition and/or interference imposed by MgDNA, was able to quantitate gastric tissue-colonizing Hp, segregating mice inoculated with low vs. high doses of Hp, as well as those immunized from controls.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Mehdi Alikhani
HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran


| Ebrahim Shafaie
HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran


| Esmat Mirabzadeh Ardakani
Animal Sciences Lab, Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran


| Maryam Esmaeili
HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran


| Samaneh Saberi
HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran


| Mojgan Hatefi
HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran


| Marjan Mohammadi
HPGC Research Group, Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-739&slc_lang=other&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/125/article-125-1498986.pdf
کد مقاله (doi)
زبان مقاله منتشر شده other
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده مقاله کوتاه
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات