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International Journal of Organ Transplantation Medicine، جلد ۱۰، شماره ۲، صفحات ۰-۰
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عنوان انگلیسی Comparison of Human Mesenchymal Stem Cells Derived from Various Compartments of Human Adipose Tissue and Tunica Adventitia Layer of the Arteries Subsequent to Organ Donation
چکیده انگلیسی مقاله Background: Mesenchymal stem cells are one of the most interesting cell sources used in regenerative medicine. Objective: In the present study, we isolated and characterized the mesenchymal stem cells from various compartments of human adipose tissue and tunica adventitia layer of the arteries. Methods: Tissue explant culture was done from various compartments of the human adipose tissue and tunica adventitia layer of the arteries, including adipose tissue far from the vessels, perivascular tissues that are completely attached to the vessels, and tunica adventitia layer of the arteries. After the cell culture, characterization of the cells was determined at 3 rd –5 th passages. Flow cytometry was performed for antigen expression analysis of CD34, CD45, CD44, CD90, CD29, CD73, and CD105. For the evaluation of cell differentiation potential, adipogenic and osteogenic differentiation was conducted under appropriate protocols. Results: The cells were positive for CD44, CD90, CD29, and CD73 and negative for CD34, CD45, and CD105. Adipogenic and osteogenic differentiation potentials were different among the cells from various compartments. The cells derived from perivascular tissue demonstrated better adipogenic and osteogenic differentiation. Conclusion: It is essential to characterize the cells from different tissues and compartments for different purposes in regenerative medicine.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | M Kaviani
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| N Azarpira
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| M Hossein Aghdaie
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| E Esfandiari
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| B Geramizadeh
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| S Nikeghbalian
Department of Transplant, Shiraz University of Medical Sciences, Shiraz, Iran


| M Dehghani
Department of Transplant, Shiraz University of Medical Sciences, Shiraz, Iran
نشانی اینترنتی http://www.ijotm.com/ojs/index.php/IJOTM/article/view/537
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Articles
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