این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Medical Physics، جلد ۱۵، شماره Special Issue-۱۲th. Iranian Congress of Medical Physics، صفحات ۴۴۵-۴۴۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The Bystander Effects of Ultraviolet Radiation and Silver Nanoparticles on the H2AX gene expression in TK6 Cells
چکیده انگلیسی مقاله Introduction: The radiation induced bystander effect (BSE) is the induction of biological changes in unexposed cells, by signals transmitted from bystander cells that cause the spread of radiation toxicity to adjacent or far tissues. In addition, reactive oxygen species (ROS) and reactive nitrogen species (RNS) such as cytokines are involved in mediating mechanisms in bystander effect through unknown factors. Bystander effects occur due to stressors, such as ionizing radiation, Ultra violet Radiation (UVR) and chemotherapy drugs, which include a wide range of biological processes, such as DNA damage, cell death, apoptosis, cell survival reduction, delayed and premature mutations, and Micronuclei formation. Silver nanoparticles (Ag NPs) are widely used in electronics, bio-sensing, food industry, paints, sunscreens and medical devices. Due to the widespread use of these particles, the toxicological risks of Ag NPs must be determined for effective and safe use. The aim of this study was to investigate the possibility of increasing bystander effect on TK6 human lymphoblastoid cell line, which were exposed to UVC and Ag NPs. Materials and Methods: The studied groups included Control, UVR, Ag NPs, UVR+ Ag NPs, BSE of UVR, BSE of UVR + Ag NPs. TK6 cells were cultured in 24-well plates. After 24hrs Cells were exposed to Ag NPs (10μg/ml) for 1hr. Then they were exposed to UVR and after irradiation to determining the bystander effect, the irradiated cells medium was transferred to non-irradiated cells. Expression level of H2AX mRNAs were examined by relative quantitative real-time polymerase chain reaction (PCR). Results: The results showed that there was a significant difference in the mean expression level of H2AX genes of the UV group compared to the control group, control with Ag NPs, BSE of UV with BSE of UV + Ag Nps. There were not differences between control and BSE of UV. Conclusion: Our findings demonstrate the gene expression of H2AX increased in UVR, Ag NPs, UVR + Ag NPs, groups significantly. In bystander groups UVR + Ag NPs decreased the H2AX gene expression in comparison to control group.
کلیدواژه‌های انگلیسی مقاله UV Radiation, Bystander Effect, Ag nanoparticle, H2ax gene
نویسندگان مقاله | Zohreh Eftekhari
Student research committee, Shiraz University of Medical Sciences, Shiraz, IRAN Department of Radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IRAN


| Reza Fardid
Department of Radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IRAN Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, IRAN


| Abbas Behzad Behbahani
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IRAN
نشانی اینترنتی http://ijmp.mums.ac.ir/article_13136.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Conference Proceedings
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات