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Iranian Journal of Basic Medical Sciences، جلد ۲۲، شماره ۷، صفحات ۷۵۹-۷۶۵
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عنوان انگلیسی Rhopalurus junceus scorpion venom induces antitumor effect in vitro and in vivo against a murine mammary adenocarcinoma model
چکیده انگلیسی مقاله Objective(s): In Cuba the endemic scorpion species Rhopalurus junceus has been used in traditional medicine for cancer treatment and related diseases. However there is no scientific evidence about its therapeutic potential for cancer treatment. The aim of the study was to determine the antitumor effect of scorpion venom against a murine mammary adenocarcinoma F3II. Materials and Methods: The cytotoxic activity was determined by MTT assay with venom concentrations ranging from 0.1–1 mg/ml. Apoptosis was determined by RT-PCR and flow cytometry. Toxic effect in healthy animals and tumor growth kinetics in F3II bearing-mice were evaluated by using scorpion venom doses (0.2; 0.8; 3.2 mg/kg) after one and ten injections respectively by the intraperitoneal route. Results: Scorpion venom induced a significant cytotoxic effect (P< 0.05) in F3II cells in a concentration-dependent manner. The cell death event involves the apoptotic pathway due to up-regulation of pro-apoptotic genes (p53, bax), down-regulation of antiapoptotic gene (bcl-2), and 33% of Annexin V+/PI- cells at early apoptosis and 10.21% of Annexin V+/PI+ cells at late apoptosis. Scorpion venom induced significant inhibition of tumor progression (P< 0.05) in F3II bearing-mice in a dose-dependent manner. The antitumor effect was confirmed due to dose-dependent reduction of Ki-67 and CD31 proteins present in tumor tissue. Conclusion: Evidence indicates that scorpion venom can be an attractive natural product for deep investigation and developing a novel therapeutic agent for breast cancer treatment.
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نویسندگان مقاله | Alexis Díaz-García
Research Department, Laboratories of Biopharmaceuticals and Chemistries Productions (LABIOFAM), Havana, Cuba


| Jenny Laura Ruiz-Fuentes
Microbiology Department, Tropical Medicine Institute “Pedro Kouri”, Havana, Cuba


| Yahima Frión-Herrera
Department of Pharmaceutical Science, Padova University, Italy


| Arianna Yglesias-Rivera
Research Department, Laboratories of Biopharmaceuticals and Chemistries Productions (LABIOFAM), Havana, Cuba


| Yanelis Riquenez Garlobo
Research Department, Laboratories of Biopharmaceuticals and Chemistries Productions (LABIOFAM), Havana, Cuba


| Hermis Rodríguez Sánchez
Microbiology Department, Tropical Medicine Institute “Pedro Kouri”, Havana, Cuba


| Juan C Rodríguez Aurrecochea
Investigation Department, Laboratory of Experimental pathology, Oncology and Radiobiology National Institute, Havana, Cuba


| Ledys X López Fuentes
Laboratory of pathology, Tropical Medicine Institute “Pedro Kouri”, Havana, Cuba
نشانی اینترنتی http://ijbms.mums.ac.ir/article_13100.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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