این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Biotechnology، جلد ۱۱، شماره ۱، صفحات ۳۲-۴۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Characterization and Potentials of Indigenous Oil-Degrading Bacteria Inhabiting the Rhizosphere of Wild Oat (Avena Fatua L.) in South West of Iran.
چکیده انگلیسی مقاله Background: The contamination of ecosystem with petroleum and its derivatives is con‌sidered as one of the most crucial environmental threat in Iran. Application of micro‌organisms has been demonstrated as an appropriate and more practical alternative to clean-up petroleum hydrocarbons in the contaminated environments. Objectives: The objectives of this study were isolating rhizosphere-inhabiting indig‌enous oil-degrading bacteria in wild oat grown in petroleum-polluted areas and in vitro evaluating the efficiency of oil biodegradability by microbial isolates. Materials and Methods: Bacteria were isolated from rhizosphere of wild oat grown on contaminated sites in Khuzestan and were identified based on 16S rRNA gene sequenc‌ing. The catabolic genes were detected using PCR and hybridization analysis. Hydrocar‌bon degradation in liquid culture was evaluated by gas chromatography-mass spec‌trometry (GC-MS). Results: 23 indigenous oil-degrading bacterial strains were isolated from the wild oat rhizosphere, grown in severely oil contaminated soil in Khuzestan. Based on 16S rRNA gene sequence analyses, isolated strains were classified to Genera Acinetobacter, Pseudo‌monas, Enterobacter, Stenotrophomonas, Bacillus, Achromobacter, Ochrobactrum, Paenibacil‌lus, Microbacterium, Curtobacterium and Sphingobacterium. Catabolic genes alkM, alkB and xylE, responsible for biodegradation of the alkanes and aromatic petrochemical com‌pounds were detected in bacterial community inhabiting rhizosphere of the wild oat. The GC-MS analysis indicated that consortium of these bacteria was capable of reducing crude petroleum in the liquid culture by 40.5%, after 10 days. The results of the present study revealed the adaptability of microbes to the rhizospheric area and subsequently their great potential to be exploited for cleaning up hydrocarbon contaminated sites. Conclusions: This study might be an important step towards the development of a phy‌toremediation strategy in the South of Iran.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله Saeideh Rajaei |
Soil Science Department, Faculty of Agriculture, Shahrekord University, Shahrekord, IR Iran

Seyed Mahdi Seyedi |
Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, IR Iran

Fayez Raiesi |
Soil Science Department, Faculty of Agriculture, Shahrekord University, Shahrekord, IR Iran

Behrouz Shiran |
Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrekord University, , Shahrekord, IR Iran

Jamshid Raheb |
Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, IR Iran

نشانی اینترنتی http://www.ijbiotech.com/article_7197_befc9cd5a5187954de33d093ad06bf95.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/441/article-441-1549972.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات