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Iranian Journal of Microbiology، جلد ۱۱، شماره ۲، صفحات ۹۸-۱۰۷
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عنوان انگلیسی Molecular analysis and antimicrobial resistance pattern of distinct strains of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Iran
چکیده انگلیسی مقاله Background and Objectives: Colonization of Pseudomonas aeruginosa in Cystic Fibrosis (CF) patients may lead to severe pulmonary disease and death. Different characteristics of P. aeruginosa from these patients were determined in the present study. Materials and Methods: Antimicrobial susceptibility and AmpC-overproduction were determined. The β-lactamase genes were detected by PCR and the oprD gene was sequenced in some of the carbapenem resistance isolates. Distribution of exo genes was determined by PCR. Cytotoxicity of Exo effector proteins was measured using A549 cells. Biofilm production was determined by microtiter plate assay. Random amplified polymorphic DNA (RAPD) –PCR was performed for molecular analysis. Results: Polymyxin B, piperacillin/tazobactam and meropenem were the most active antibiotics and 9.6% of isolates were ampC overproducers. The prevalence of blaVEB, blaOXA, blaVIM, and blaPER genes were as follow: 22.7%, 3.75%, 6.25% and 3.75%, respectively. A high proportion (83.5%) of isolates was able to produce biofilm. The exoT gene was present in all isolates while exoU was present in about 35% of them. RAPD-PCR revealed 49 patterns among 78 tested isolates in which 34 patterns were detected once. Conclusion: Biofilm formation ability and relatively high frequency of exoS may contribute to the persistence of bacteria within lungs of CF patients. Some characteristics of isolates recovered from a single patient after several sampling procedures were similar, while others lacked resemblance.
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نویسندگان مقاله | Mohammad Emaneini
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Davood Kalantar-Neyestanaki
Department of Microbiology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran


| Leila Jabalameli
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran


| Mojtaba Hashemi
Department of Pediatric Gastroenterology, School of Medicine, Arak University of Medical Sciences, Arak, Iran


| Reza Beigverdi
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Fereshteh Jabalameli
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/1887
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