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Iranian Journal of Microbiology، جلد ۱۱، شماره ۲، صفحات ۱۸۱-۱۸۶
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عنوان انگلیسی A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification
چکیده انگلیسی مقاله Background and Objectives: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3´ to 5´ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs). This study was aimed to express and purify Pfu DNA polymerase in a bacterial expression system under a simple purification method. Materials and Methods: Pfu polymerase gene sequence, derived from Pyrocuccus furiosus (Pfu) genomic DNA, was cloned and overexpressed in E. coli BL21 (DE3) pLysS. Upon overexpression, bacterial lysate containing the Pfu DNA polymerase was heated at 94°C for 5 minutes. Pfu DNA polymerase having high thermal stability was retained while the other bacterial proteins were denatured. The resulting thermo stable Pfu DNA polymerase was separated from the other debris of the denatured proteins by simple centrifugation. Results: The enzymatic activity of the resulting Pfu DNA polymerase was estimated by comparing with the commercial Pfu DNA Polymerases. An estimated 50000 units of functional Pfu DNA polymerase was produced from a 400 ml culture. Conclusion: The in-house produced Pfu DNA Polymerase could be used for routine amplification that requires high-fidelity such as cloning and DNA sequencing.
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نویسندگان مقاله | Prabu Sankar
Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, Malaysia


| Marimuthu Citartan
Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, Malaysia


| Aminah Siti
Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, Malaysia


| Boris V. Skryabin
Medical Faculty (TRAM), University of Muenster, Muenster, Germany


| Timofey S. Rozhdestvensky
Medical Faculty (TRAM), University of Muenster, Muenster, Germany


| Goot Khor
Centre of Preclinical Science Studies, Faculty of Dentistry, Universiti Teknologi MARA, Sungai Buluh Campus, Selangor, Malaysia


| Thean Tang
Advanced Medical & Dental Institute (AMDI), Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, Malaysia
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/1929
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/91/article-91-1566391.pdf
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