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Iranian Journal of Medical Sciences، جلد ۳۹، شماره March Supplement، صفحات ۱۹۶-۲۰۲
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عنوان انگلیسی Expression Status of UBE2Q2 in Colorectal Primary Tumors and Cell Lines
چکیده انگلیسی مقاله Background: Activation of the ubiquitin-proteasome pathway in various malignancies, including colorectal cancer, is established. This pathway mediates the degradation of damaged proteins and regulates growth and stress response. The novel human gene, UBE2Q2, with a putative ubiquitin-conjugating enzyme activity, is reported to be overexpressed in some malignancies. We sought to investigate the expression levels of the UBE2Q2 gene in colorectal cell lines as well as in cancerous and normal tissues from patients with colorectal cancer. Methods: Levels of UBE2Q2 mRNA in cell lines were assessed by Real-Time PCR. Western blotting was employed to investigate the levels of the UBE2Q2 protein in 8 colorectal cell lines and 43 colorectal tumor samples. Results: Expression of UBE2Q2 was observed at the level of both mRNA and protein in colorectal cell lines, HT29/219, LS180, SW742, Caco2, HTC116, SW48, SW480, and SW1116. Increased levels of UBE2Q2 immunoreactivity was observed in the 65.11% (28 out of 43) of the colorectal carcinoma tissues when compared with their corresponding normal tissues. Difference between the mean intensities of UBE2Q2 bands from cancerous and normal tissues was statistically significant at P< 0.001 (paired t test). Conclusion: We showed the expression pattern of the novel human gene, UBE2Q2, in 8 colorectal cell lines. Overexpression of UBE2Q2 in the majority of the colorectal carcinoma samples denotes that it may have implications for the pathogenesis of colorectal cancer.
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نویسندگان مقاله Sayed Mohammad Shafiee |
Department of Biochemistry-Recombinant Protein Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Atefeh Seghatoleslam |
Department of Biochemistry-Recombinant Protein Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Mohsen Nikseresht |
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

Seyed Vahid Hosseini |
Colorectal Research Center, Shahid Faghihi Hospital, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

Mahvash Alizadeh-Naeeni |
Department of Internal Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Akbar Safaei |
Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Ali Akbar Owji |
Department of Biochemistry-Recombinant Protein Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, and Endocrine and Metabolism Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

نشانی اینترنتی http://ijms.sums.ac.ir/article_44113_6a3c6d053b12dc73f4645ce118af0e83.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/147/article-147-1611507.pdf
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