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Journal of Arthropod-Borne Diseases، جلد ۱۳، شماره ۲، صفحات ۱۴۵-۱۵۲
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عنوان انگلیسی Molecular Analysis of Aquaglyceroporin 1 Gene in Non-Healing Clinical Isolates Obtained from Patients with Cutaneous Leishmaniasis from Central of Iran
چکیده انگلیسی مقاله Background: Regarding the antimonial-resistant of Leishmania spp., understanding of related mechanism is neces­sary. One of the most important involved molecules is aquaglyceropin1 (AQP1). The aim of this study was molecu­lar analysis of AQP1 gene from antimonial-resistant clinical isolates and its expression. Methods: Overall, 150 patients with cutaneous leishmaniasis referring to the reference laboratories of Yazd and Varzaneh,, located 105km southeast of Isfahan and 240km away from Yazd, were assessed from Jun 2015 to Dec 2017. After sampling, staining was done and evaluated for Leishman by microscope. Samples were collected in RNAlater solution for gene expression analysis in non-healing isolates. DNA extraction was performed from each slide with Leishman body. All patients with L. major isolates detected by ITS1-PCR-RFLP were followed for find­ing the resistant isolates, consequence of molecular characterization of AQP1 using PCR-RFLP. Gene expression of AQP1 from all resistant isolates was assessed in comparison with the one in a sensitive isolate. Statistical analysis was done using SPSS. The significance level was considered ≤0.05. Results: Five isolates were detected as antimonial resistant. Molecular detection and identification were appeared that all were L. major. The molecular characterization of AQP1 showed G562A mutation. Gene expression of AQP1 in resistant isolates showed 1.67 fold higher than the sensitive isolate. Conclusion: We reported a new point mutation of G562A in AQP1 gene involved in molecular mechanism in re­sistant isolates.
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نویسندگان مقاله | Yasaman Alijani
Research Center for Food Safety and Health, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran


| Saeedeh Sadat Hosseini
Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran


| Salman Ahmadian
Research Center for Food Safety and Health, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran, Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran


| Sonia Boughattas
Biomedical Research Center, Qatar University, Doha, Qatar


| Gilda Eslami
Research Center for Food Safety and Health, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran, Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran


| Shadi Naderian
Department of Statistics and Epidemiology, School of Public Health, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran


| Vahid Ajamian
Research Center for Food Safety and Health, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran, Department of Parasitology and Mycology, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/802
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