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Journal of Arthropod-Borne Diseases، جلد ۱۳، شماره ۲، صفحات ۲۰۶-۲۱۳
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عنوان انگلیسی Identification of Alleles in the MSP1 Gene Related to Complicated Malaria in Patients Infected with Plasmodium falciparum in Southeast of Iran
چکیده انگلیسی مقاله Background: To overcome human malaria problem several solutions have been employed including extensive studies in the field of Plasmodia relevant antigens. The aim of this study was to determine allelic variation in the MSP1 gene of Plasmodium falciparum among some falciparum malaria-infected patients in Southeastern Iran. Methods: Twenty P. falciparum positive cases were enrolled from Sistan and Baluchistan Province, southeastern Iran in 2013–15. From each case, 1.5ml of peripheral blood was collected into EDTA contained tubes. Thick and thin blood smears were stained with standard Giemsa stain and were checked with conventional microscopical method. DNA was extracted from blood samples and amplification of block 2 MSP1 was performed using specific primers. Gel electropho­resis was done and results showed some amplification fragments corresponding to block 2 regions of Pf MSP1 gene. Finally, four samples from different allelic types were sent for sequencing process. Results: Fragments were different in size, so classified into six allelic types as kinds of 1–6 based on happening fre­quencies. Digestion of PCR products revealed two sub allelic types (A and B) within allelic types 2 and 3, but not in al­lelic types 1, 4, 5 and 6. Twenty percent of samples were sent for sequencing. Sequence alignment showed 78.95% to 91.83% identity between samples. Conclusion: Identity between samples and phylogenetic tree revealed that there is an extensive diversity range among isolates. Fifty percent of the isolates were under the risk of complicated malaria. Two of these patients (10%) needed special care and recovery was obtained after getting hospital services.
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نویسندگان مقاله | Bentol Hoda Habibi-Shorkaei
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Afsaneh Motevalli-Haghi
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Mehdi Nateghpour
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran, Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran


| Leila Farivar
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran,


| Homa Hajjaran
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran,


| Soudabeh Etemadi
Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/1142
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