| چکیده انگلیسی مقاله |
Background: Endometriosis is a common gynecological disease in which oxidative stress is a potential factor. Caffeine and caffeic acid are present in various foods and beverages with anti-oxidant, anti-inflammatory, and anti-carcinogenic properties. In this study, we aimed to investigate the ameliorative effects of caffeine, caffeic acid, and caffeine+caffeic acid treatments on oxidative stress in ectopic endometrial cells taken from patients and eutopic ones from women without endometriosis.Methods: In this experimental study, eutopic and ectopic endometrial cells were obtained from biopsies of women free of disease (n=10) and patients with endometriosis (n=10) who referred to Shiraz reference hospitals (2017-2018). Both eutopic and ectopic endometrial cells were divided into four groups: Treated with caffeine, with caffeic acid, with caffeine+caffeic acid, and the control. Also, antioxidant enzyme activities and the levels of glutathione (GSH) and malondialdehyde (MDA) were determined in each group. The data were analyzed using independent sample t test and one-way ANOVA followed by Tukey post-hoc test.Results: Caffeic acid, but not caffeine treatment demonstrated a decrease in MDA level (P< 0.001) as well as an increase in GSH level (P< 0.001) and antioxidant enzyme activities in ectopic endometrial cells. Also, the treatment of the cells with caffeine+caffeic acid caused similar effects as those ectopic cells treated with caffeic acid. Conclusion: According to the findings of the present study, caffeic acid reduced oxidative stress which may alleviate the complications associated with endometriosis. However, more investigations are needed for evaluating the efficiency and safety of caffeic acid. |
| نویسندگان مقاله |
Navid Jamali |
Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
Zohreh Mostafavi-Pour |
Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Maternal-Fetal Medicine Research
Center, Shiraz University of Medical
Sciences, Shiraz, Iran
Fatemeh Zal |
Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Maryam Kasraeian |
Maternal-Fetal Medicine Research
Center, Shiraz University of Medical
Sciences, Shiraz, Iran; and Department of OB/GYN, School of
Medicine, Shiraz University of Medical
Sciences, Shiraz, Iran
Tahereh Poordast |
Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; and Department of OB/GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Fatemeh Ramezani |
Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
Razieh Zare |
Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
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