این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Molecular Biology Research Communications، جلد ۸، شماره ۲، صفحات ۵۹-۶۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Phylogenetic diversity and cross-inoculation of indigenous isolated Bradyrhizobium from nodules of peanut in Liaoning province of China
چکیده انگلیسی مقاله Arachis hypogaea. L is a legume of economic importance, which is nodulated by Bradyrhizobium, a slow-growing bacteria. However there is no well characterization of this rhizobia in many areas of China. In the present study, cross-inoculation experiments were performed in cowpea and soybean. The isolated bacteria strains were characterized physiologyically, biochemically and identified through 16S rDNA sequence analysis showing that it belongs to Bradyrhizobium japonicum. The genetic diversity of the seventeen isolated strains were assessed through PCR-RFLP of 16S rDNA and 16S-23S rDNA IGS region. Cross inoculation test indicated that isolates could nodulate cowpea but not soybean. The cluster analysis based on physiological and biochemical characteristics showed the lower correlation between isolates and sites. The isolates were grouped into four clusters based on 16S rDNA gene sequence analysis. Thirteen polymorphisms were variable across all observations in 16S rDNA RFLP and six different IGS types from isolates. The results implies that there was some association between geographical factor and phylogenetic diversity of indigenous Bradyrhizobium isolates.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله Hongzhi Bai |
College of Land and Environment, Shenyang Agricultural University, Shenyang, China

Yanhua Zhang |
College of Land and Environment, Shenyang Agricultural University, Shenyang, China

Haiqiu Yu |
College of Agronomy, Shenyang Agricultural University, Shenyang, China

Muhammad Irfan |
Department of Biotechnology, University of Sargodha, Sargodha Pakistan

Huang Yuqian |
College of Land and Environment, Shenyang Agricultural University, Shenyang, China

Han Mei |
College of Land and Environment, Shenyang Agricultural University, Shenyang, China

Jinfeng Yang |
College of Land and Environment, Shenyang Agricultural University, Shenyang, China

Ning Liu |
College of Land and Environment, Shenyang Agricultural University, Shenyang, China

Hui Wang |
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, China

Xiaori Han |
College of Land and Environment, Shenyang Agricultural University, Shenyang, China

نشانی اینترنتی http://mbrc.shirazu.ac.ir/article_5201_e611054a6e1a33b78ee3ea3e65712547.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/526/article-526-1674829.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات