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Molecular Biology Research Communications، جلد ۸، شماره ۲، صفحات ۷۹-۸۹
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عنوان انگلیسی Immunoregulatory impact of human mesenchymal-conditioned media and mesenchymal derived exosomes on monocytes
چکیده انگلیسی مقاله Mesenchymal stem cells (MSCs) are well known due to their immunomodulatory effect, but the exact mechanisms have not been defined. Several studies demonstrated that the exerted immunoregulatory effect of these cells could be mediated by paracrine factors to illustrate, cytokines, chemokine, and among which, extracellular vesicles are one of them to play a crucial role. Moreover, it is assumed that extracellular vesicles are an essential player in intracellular communication by transferring their component. In this respect, the efficiency of conditioned media and exosomes was compared to illustrate a practical approach to cell-free based therapies. In the current study, we investigated the effect of both MSCs conditioned media (MSC-CM) and MSCs-derived exosomes on the expression of pro-inflammatory and anti-inflammatory cytokines in peripheral blood mononuclear cells (PBMCs). In this regard, isolated PBMCs were treated with MSC-CM and MSC-derived exosome as separated groups. Expression of inflammatory and anti-inflammatory markers was evaluated by Real-time PCR and ELISA. The immunoregulatory effect of MSC-CM on pro-inflammatory and anti-inflammatory genes, such as IL-12b, iNOS, EGR-2, IL-10 with an exception in case of IL-6 was more significant. Whereas in protein levels IL-10 showed the most substantial difference in exosome treated groups. It could be assumed that MSC-CM has more immunoregulatory impact on monocyte in contrast with exosomes.Taken together, by considering the recent approaches to cell-free therapy and the immunoregulatory impact of MSCs, yet relatively little is known about the efficacy of human-MSC-CM and secreted exosome compared with each other.
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نویسندگان مقاله Samaneh Tokhanbigli |
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Kaveh Baghaei |
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Ali Asadirad |
Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Seyed Mahmoud Hashemi |
Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Hamid Asadzadeh-Aghdaei |
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mohammad Reza Zali |
Gastroenterology and Liver Diseases Research center, Research institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

نشانی اینترنتی http://mbrc.shirazu.ac.ir/article_5252_b59ab3cb04c59827d037183baafe7a40.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/526/article-526-1674831.pdf
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