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JCR 2016
جستجوی مقالات
پنجشنبه 27 آذر 1404
Iranian Journal of Biotechnology
، جلد ۱۷، شماره ۲، صفحات ۴۶-۵۴
عنوان فارسی
Cell Suspension Culture of Plumbago europaea L. Towards Production of Plumbagin
چکیده فارسی مقاله
Background:
Plumbagin is as an important bioactive secondary metabolite found in the roots of
Plumbago
spp. The only one species,
Plumbago europaea
L., grows wild in Iran. The therapeutic use of plumbagin is limited due to its insufficient supply from the natural sources as the plants grow slowly and take several years to produce quality roots.
Objectives:
To develop an efficient protocol for the establishment of callus and cell suspension cultures of
P. europaea
and to evaluate production of plumbagin in callus and cell suspension cultures of
P. europaea
for the first time.
Material and Methods:
Stems and leaves explants were cultured on agar solidified (7% w/v) MS media, supplemented with different combination of 2, 4-D and Kin or 6-Benzylaminopurin (BA) for callus induction. The rapid growing calli were cultured in liquid Murashige and Skoog (MS) media in agitated condition for establishing cell suspension cultures of
P. europaea
. Moreover, the effects of light and dark conditions on the cell growth, cell viability and plumbagin production in cell suspension cultures of
P. europaea
were assessed.
Results:
Friable calli were successfully induced using stem segments of
P. europaea
in semisolid MS medium supplemented with 1 mg.L
-1
2, 4-Dichlorophenoxy acetic acid (2, 4-D) and 0.5 mg.L
-1
of kinetin (Kin). Optimal cell growth was obtained when the cells were grown in MS liquid media supplemented with 1 mg.L
-1
2, 4-D and 0.5 mg.L
-1
kinetin with an initial cell density of ~3×10
5
cellsper ml incubated in the dark at 25 ± 1 °C. Growth curve revealed that the maximum cell growth rate (14.83×10
5
cellsper ml) achieved on the day 18 and the highest plumbagin content (0.9 mg.g
-1
Dry Cell Weight (DCW)) in the cells was obtained at the late exponential phase under dark condition which determined by High Performance Liquid Chromatography (HPLC) technique. Based on the obtained results, cell viability remained around 82.73% during the 18 days of cell culture in darkness. These suspension cultures showed continuous and stable production of plumbagin.
Conclusions:
Our study suggests that cell suspension cultures of
P. europaea
represent an effective system for biosynthesis and production of plumbagin as a valuable bioactive compound.
کلیدواژههای فارسی مقاله
Cell Survival،، Light،، Plumbagin،
عنوان انگلیسی
Cell Suspension Culture of Plumbago europaea L. Towards Production of Plumbagin
چکیده انگلیسی مقاله
Background:
Plumbagin is as an important bioactive secondary metabolite found in the roots of
Plumbago
spp. The only one species,
Plumbago europaea
L., grows wild in Iran. The therapeutic use of plumbagin is limited due to its insufficient supply from the natural sources as the plants grow slowly and take several years to produce quality roots.
Objectives:
To develop an efficient protocol for the establishment of callus and cell suspension cultures of
P. europaea
and to evaluate production of plumbagin in callus and cell suspension cultures of
P. europaea
for the first time.
Material and Methods:
Stems and leaves explants were cultured on agar solidified (7% w/v) MS media, supplemented with different combination of 2, 4-D and Kin or 6-Benzylaminopurin (BA) for callus induction. The rapid growing calli were cultured in liquid Murashige and Skoog (MS) media in agitated condition for establishing cell suspension cultures of
P. europaea
. Moreover, the effects of light and dark conditions on the cell growth, cell viability and plumbagin production in cell suspension cultures of
P. europaea
were assessed.
Results:
Friable calli were successfully induced using stem segments of
P. europaea
in semisolid MS medium supplemented with 1 mg.L
-1
2, 4-Dichlorophenoxy acetic acid (2, 4-D) and 0.5 mg.L
-1
of kinetin (Kin). Optimal cell growth was obtained when the cells were grown in MS liquid media supplemented with 1 mg.L
-1
2, 4-D and 0.5 mg.L
-1
kinetin with an initial cell density of ~3×10
5
cellsper ml incubated in the dark at 25 ± 1 °C. Growth curve revealed that the maximum cell growth rate (14.83×10
5
cellsper ml) achieved on the day 18 and the highest plumbagin content (0.9 mg.g
-1
Dry Cell Weight (DCW)) in the cells was obtained at the late exponential phase under dark condition which determined by High Performance Liquid Chromatography (HPLC) technique. Based on the obtained results, cell viability remained around 82.73% during the 18 days of cell culture in darkness. These suspension cultures showed continuous and stable production of plumbagin.
Conclusions:
Our study suggests that cell suspension cultures of
P. europaea
represent an effective system for biosynthesis and production of plumbagin as a valuable bioactive compound.
کلیدواژههای انگلیسی مقاله
Cell Survival, Light, Plumbagin
نویسندگان مقاله
Mina Beigmohammadi |
Faculty of Natural Science, University of Tabriz, Tabriz, Iran
Ali Movafeghi |
Faculty of Natural Science, University of Tabriz, Tabriz, Iran
Ali Sharafi |
Zanjan Applied Pharmacology Research Center, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
Samineh Jafari |
Pharmacognosy Department, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
Hossein Danafar |
Zanjan Pharmaceutical Biotechnology Research Center, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
نشانی اینترنتی
http://www.ijbiotech.com/article_85187_454c7ae247a8bdcdf5f6b15d8eda2190.pdf
فایل مقاله
اشکال در دسترسی به فایل - ./files/site1/rds_journals/441/article-441-1681178.pdf
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