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Iranian Biomedical Journal، جلد ۲۳، شماره ۵، صفحات ۳۲۴-۳۲۹
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عنوان انگلیسی The Lentiviral Vector Pseudotyped by Modified Rabies Glycoprotein Does Not Cause Reactive Gliosis and Neurodegeneration in Rat Hippocampus
چکیده انگلیسی مقاله Background: A human immunodeficiency virus type 1 (HIV-1)-based lentiviral vector (LV) pseudotyped by a variant of rabies envelope glycoprotein, FUG-B2, has previously been prepared and used in transfection of hippocampal CA1 ("Cornu Ammonis" area 1) neurons. This study aimed to verify reactive gliosis and neuronal damage after injection of the vector into the rat hippocampus. Methods: HEK 293T cells were transfected with transfer (fck-Jaws-GFP-ER2), envelope (FUG-B2), and packaging (pMDLg/pRRE, pRSV-Rev) plasmids, and the vector was injected into CA1 of the rat hippocampus. After one week, transduction efficiency, and the number of neuronal and astroglial cells were determined in CA1 and CA3 by double staining of the brain slices. Results: Hippocampal cells were successfully transfected as 92.7% of CA1 and 95.8% of CA3 neuronal cells expressed GFP. The frequency of neuronal and astroglial cells in CA1 and CA3 of the vector-injected rats remained unchanged compared to those in the control and the saline-injected rats. Furthermore, no morphological change was found in hippocampal astrocytes and neuronal cells. Conclusion: The HIV-1-based LV pseudotyped by FUG-B2 is safe and does not cause neuroinflammation and neuronal loss once directly delivered into the rat hippocampus. 
کلیدواژه‌های انگلیسی مقاله Astrocytes, CA3 region, Rat Rabies virus glycoprotein
نویسندگان مقاله | Mostafa Farzaneh
Department of Pharmacology, Science and Research Branch, Islamic Azad University, Tehran, Iran.


| Mohammad Sayyah
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.


| Hamid Reza Eshraghi
Department of Pharmacology, Science and Research Branch, Islamic Azad University, Tehran, Iran.


| Negar Panahi
Department of Pharmacology, Science and Research Branch, Islamic Azad University, Tehran, Iran.


| Hadi Mirzapourdelavar
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.


| Hamid Gholami Pourbadie
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-776&slc_lang=other&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/125/article-125-1687391.pdf
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