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Journal of Crop Protection، جلد ۸، شماره ۴، صفحات ۳۷۹-۳۸۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Detection and molecular characterization of tomato big bud disease in Qazvin province
چکیده انگلیسی مقاله Some samples were collected from tomato fields in Qazvin from tomato plants with big bud symptoms such as plant droop and purplish vein under the leaf, enlarged and sac-like pistils and malformed buds. DNA was extracted from the veins and vascular tissues of the plant with CTAB-based methods. In symptomatic plants, DNA fragments of 1800 and 1200bp were amplified by PCR using P1/P7, R16F2n/R16R2 primers. Restriction fragment length polymorphism (RFLP) analysis of nested R16F2n/R16R2 primed PCR product (1200bp) showed that the tomato big bud phytoplasma from Qazvin (TOM-Qazvin) is a member of clover proliferation (16SrVI). Phylogenetic analysis of 16SrRNA and putative restriction site analysis of the R16F2n/R16R2 primed sequence classified TOM phytoplasma in clover proliferation (16SrVI) group and belonged to subgroup 16SrVI-A. Virtual RFLP by using 1200bp sequencing of 16SRNA and 17 restriction enzymes confirmed that TOM-Qazvin belonged to the subgroup 16SrVI-A and16SrVI group. To our knowledge, this is the first report of tomato big bud disease in Qazvin province.
کلیدواژه‌های انگلیسی مقاله big bud, iphyclassifier, RFLP, tomato, phytoplasma
نویسندگان مقاله | Abbas Davoodi
Department of Plant Protection, Faculty of Agriculture, Zabol University, Zabol, Iran.


| Naser Panjekeh
Department of Plant Protection, Faculty of Agriculture, Zabol University, Zabol, Iran.


| Kobra Moslemkhani
Seed and Plant Certification and Registration Institute, AREEO, Karaj, Iran.


| Abdolhossein Taheri
Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
نشانی اینترنتی http://journals.modares.ac.ir/browse.php?a_code=A-10-10798-3&slc_lang=en&sid=3
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده پژوهشی اصیل
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