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International Journal of Radiation Research، جلد ۱۲، شماره ۲، صفحات ۱۱۳-۱۲۱
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عنوان انگلیسی Protective effect of ferulic acid on ionizing radiation induced damage in bovine serum albumin
چکیده انگلیسی مقاله Background: Ionizing radiation causes deleterious effects on living system mainly due to oxidative damages of macromolecules and protein is the major target due to its abundance. The aim of this study was to investigate the effects of ionizing radiation induced changes in the molecular properties of bovine serum albumin (BSA) its secondary and tertiary structures, degradation, cross linking and radioprotective role of ferulic acid, a natural antioxidant on these radiation induced changes. Materials and Methods: This study was carried out to investigate the gamma radiation induced oxidative, structural damage of BSA and radioprotective efficacy of ferulic acid through SDS-PAGE, DTNB assay, DNPH assay, FOX assay methods. Hydroxyl radical scavenging capacity of ferulic acid was estimated using 2-deoxy ribose assay. Further, radiation induced changes in the anisotropy and excitation state lifetimes of BSA were examined. Results: SDS –PAGE data suggested that the loss of protein was linearly dependent on the radiation dose. Gamma-irradiation of BSA caused the formation of protein carbonyls, hydroperoxides and loss of thiols. Ferulic acid protected the radiation induced loss of protein as well as reduced various oxidative damages. Ferulic acid protected the protein from radiation induced damages in a concentration dependent manner. Conclusion: The results provide insight into radiation induced molecular changes in the protein. Ferulic acid protected the BSA from oxidative modification caused by radiation suggesting that ferulic acid possesses strong antiradical properties. Ferulic acid is known to protect DNA, the prime target of radiation and further its ability to protect protein suggesting its ability to protect different biomolecules and therefore can be a good candidate for development radioprotector.
کلیدواژه‌های انگلیسی مقاله BSA, protein carbonyl, radioprotection, ferulic acid
نویسندگان مقاله k میشرا | k. mishra
chemical radioprotector and radiation dosimetry research group, division of radiation biosciences, institute of nuclear medicine and allied sciences, delhi-110054, india


h ojha | h. ojha
chemical radioprotector and radiation dosimetry research group, division of radiation biosciences, institute of nuclear medicine and allied sciences, delhi-110054, india


s kallepalli | s. kallepalli
chemical radioprotector and radiation dosimetry research group, division of radiation biosciences, institute of nuclear medicine and allied sciences, delhi-110054, india


a alok | a. alok
chemical radioprotector and radiation dosimetry research group, division of radiation biosciences, institute of nuclear medicine and allied sciences, delhi-110054, india


n کومار chaudhury | n kumar chaudhury
chemical radioprotector and radiation dosimetry research group, division of radiation biosciences, institute of nuclear medicine and allied sciences, delhi-110054, india
نشانی اینترنتی http://www.ijrr.com/browse.php?a_code=A-10-1-496&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Radiation Biology
نوع مقاله منتشر شده تحقیق بدیع
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