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International Journal of Radiation Research، جلد ۱۰، شماره ۱، صفحات ۱۱-۱۸
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عنوان انگلیسی Radiosensitizing effects of gemcitabine on aerobic and chronically hypoxic HeLa and MRC5 cells in-vitro
چکیده انگلیسی مقاله Background: Gemcitabine (2′, 2′-difluoro-2′- deoxycytidine, an analogue of deoxycytidine) is a relatively new drug with wide range of anti-cancer activity. In this study, radiosensitizing effects of gemcitabine was investigated on HeLa and MRC5 human originated cell lines under both chronically hypoxic and normoxic conditions using the micronucleus (MN) assay. Materials and Methods: For induction of chronic hypoxia, the cell culture flasks were saturated with N2 gas. To evaluate the radiosensitizing effects, in the presence of the non-genotoxic concentration (1ng/ml) of gemcitabine, cells were exposed to different doses (0.5, 1, 2 Gy) of X-ray in both chronically hypoxic and normoxic conditions. Results: Results showed that there was no significant difference in MN induction under chronically hypoxic and normoxic condition when using 1 ng/ml gemcitabine alone, however in the absence of drug, MN induction was significantly different in irradiated cells (P< 0.01). Radiosensitizing effects of gemcitabine in chronic hypoxic condition was greater than normoxic condition in both cell lines (P< 0.01), although more pronounced in HeLa cells. Conclusions: Radiosensitizing effects and greater dose modifying factor of gemcitabine under depleted oxygen condition is not clearly understood. It might be due to depletion of deoxynocleotides pools via inhibition of ribonucleotide reductase and mismatched nucleosides incorporation into DNA after radiation exposure. Iran. J. Radiat. Res., 2012 10(1): 11-18
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نویسندگان مقاله e حشمتی | e. heshmati
department of biophysics, faculty of biological sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

h مزدارانی | h. mozdarani
department of medical genetics, faculty of medical sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

p عبدالملکی | p. abdolmaleki
department of biophysics, faculty of biological sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

k خوشامن | k. khoshaman
department of biology, parand branch, islamic azad university, saveh highway, tehran, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

نشانی اینترنتی http://www.ijrr.com/browse.php?a_code=A-10-1-415&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Radiation Biology
نوع مقاله منتشر شده تحقیق بدیع
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