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International Journal of Radiation Research، جلد ۸، شماره ۴، صفحات ۲۴۳-۲۴۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Synthesis and biodistribution study of a chlorotoxin derivative peptide labeled with 131- iodine for tumor therapy
چکیده انگلیسی مقاله Background: Chlorotoxin is a 36-amino acid peptide found in the venom of the Leiurus quinquestriatus which blocks small-conductance chloride channels. Chlorotoxin binds preferentially to glioma cells that allow development of new methods for the treatment and diagnosis of several types of cancer. Thus chlorotoxin derivative was labeled with 131I for further investigation. Materials and Methods: A chlorotoxin derivative was synthesized on a solid phase using a standard Fmoc strategy. Labeling with iodine-131 was performed through chloramine-T method and radiochemical analysis involved sephadex G-25 and HPLC methods. The stability of radiopeptide was checked in the presence of PBS and human serum at 37 °C up to 24 h. The biodistribution was studied in mice. Results: The chemical purity of synthesized peptide as assessed by analytical RP-HPLC was 95%. Labeling of peptide resulted in a radiochemical yield of 80% with radiochemical purity of > 95% with specific activity of 0.740 GBq/μmol. Result of in vitro studies demonstrated acceptable stability of compound in human serum and PBS solution. Biodistribution data showed moderate blood clearance, with concentration of radioactivity in the kidneys, liver, intestine and stomach. Conclusion: Results indicates that the labeled Chlorotoxin derivative might be useful in determining tumor extent and also, tumor therapy of gliomas or possibily other cancers. Iran. J. Radiat. Res., 2011 8(4): 243-248
کلیدواژه‌های انگلیسی مقاله Cancer, peptide, 131I, labeling, chlorotoxin.
نویسندگان مقاله سایمون شیرمردی | s p shirmardi
1 nuclear engineering and physics, amirkabir university of technology, tehran, iran

سازمان اصلی تایید شده: دانشگاه صنعتی امیرکبیر (Amirkabir university of technology)

m شمسایی | m. shamsaei
1 nuclear engineering and physics, amirkabir university of technology, tehran, iran

سازمان اصلی تایید شده: دانشگاه صنعتی امیرکبیر (Amirkabir university of technology)

m گندمکار | m. gandomkar
nuclear science research school, nuclear sciences and technology research institute, tehran, iran


m قنادی مراغه | m ghannadi maragheh
nuclear fuel cycle research school, nuclear sciences and technology research institute, tehran, iran
نشانی اینترنتی http://www.ijrr.com/browse.php?a_code=A-10-1-375&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Radiation Biology
نوع مقاله منتشر شده تحقیق بدیع
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