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International Journal of Radiation Research، جلد ۷، شماره ۲، صفحات ۶۳-۶۸
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عنوان انگلیسی Radioresponse of human lymphocytes pretreated with boron and gadolinium as assessed by the comet assay
چکیده انگلیسی مقاله Background: Boron and gadolinium are among the nuclides that hold a unique property of being a neutron capture therapy agent. Neutron beams have often a considerable portion of gamma rays with fast neutrons. Gamma rays, as beam contaminants, can cause considerable damage to normal tissues even if such tissues do contain high boron concentrations. Materials and Methods: The modification of radioresponse in human lymphocytes pretreated with boron or gadolinium compound was studied by assessing the DNA damage using single cell gel electrophoresis (SCGE), the comet assay. The lymphocytes from the human peripheral blood were irradiated with 0, 1, 2 and 4 Gy of gamma rays from a 60Co isotopic source with or without pretreatment of boron or gadolinium compound for 10 minutes at 4oC. Post-irradiation procedures included slide preparation, cell-lysing, unwinding and electrophoresis, neutralization, staining, and analytic steps, gel electrophoresis. Results: The results indicate that pretreatment with boron compound (50 nM or 250 nM of 10B) is effective in reducing the radiosensitivity of the lymphocyte DNA. Conversely, pretreatment with gadolinium compound (50 nM) led to a dose-dependent increase in the radiosensitivity, most prominently with a dose of 4 Gy (P< 0.001). Furthermore, when the lymphocytes were pretreated with a combined mixture (1:1) of boron (250 nM) and gadolinium (50 nM) compounds, the reduced radiosensitivity was also observed. Iran. J. Radiat. Res., 2009 7 (2): 63-68
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نویسندگان مقاله j k کیم | j k kim
korea atomic energy research institute, jeongeup, 580-185, south korea


t w سونگ گو | t w park
hyundai ep, bundang, songnam, 463-811, south korea


a cebulska wasilewska | a cebulska wasilewska
the nenryk niewodniczanski institute of nuclear physics, 31-342 krakōw, poland


m نیلی | m. nili
dawnesh radiation research institute, 08007 barcelona, spain
نشانی اینترنتی http://www.ijrr.com/browse.php?a_code=A-10-1-319&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Radiation Biology
نوع مقاله منتشر شده تحقیق بدیع
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