این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
International Journal of Radiation Research، جلد ۴، شماره ۴، صفحات ۱۸۷-۱۹۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Production and evaluation of [67Ga]-DTPA-Rituximab
چکیده انگلیسی مقاله Background: In order to obtain an anti-CD20 conjugate to be used in future therapeutic studies with therapeutic radioisotopes, 67Ga-labeled antibody was prepared as a model of metal chelated immunoconjugate for preliminary dosimetric and biodistribution studies. Materials and Methods: Rituximab was labeled with [67Ga]-gallium chloride after residulation with freshly prepared cyclic DTPAdianhydride. The best results of the conjugation were obtained by the addition of 1 ml of a rituximab pharmaceutical solution (5 mg/ml, in phosphate buffer, pH=8) to a glass tube pre-coated with DTPAdianhydride (0.01 mg) at 25|o|C with continuous mild stirring for 30 min. The final isotonic 67Ga-DTPArituximab complex was checked by gel electrophoresis for radiolysis/chemolysis control. Radio-TLC was performed to ensure the formation of only one species. Preliminary in vivo studies in normal rat model were performed to determine the biodistribution of the radioimmunoconjugate up to 6 hours. Results: Radio-thin layer chromatography showed an overall radiochemical purity of 96-99% at optimized conditions (specific activity =300-500 MBq/mg, labeling efficiency 77%). Gel electrophoresis showed no protein cleavage after radiolabeling. Conclusion: Preliminary in vivo studies in normal rat model showed [67Ga]-DTPA-rituximab is a good probe for bio-dosimetry of therapeutic rituximab conjugates. Iran. J. Radiat. Res., 2007 4 (4): 187-193
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله علیرضا جلیلیان | a r jalilian
department of cyclotron amp;amp; nuclear medicine, nuclear research center for agriculture and medicine, karaj, iran


l mirsadegh | l. mirsadegh
department of biochemistry, payam-e-noor university, tehran, iran


r حاجی حسینی | r haji hosseini
department of biochemistry, payam-e-noor university, tehran, iran


s رجبی فر | s. rajabifar
department of cyclotron amp;amp; nuclear medicine, nuclear research center for agriculture and medicine, karaj, iran


f bolurinovin | f. bolurinovin
department of cyclotron amp;amp; nuclear medicine, nuclear research center for agriculture and medicine, karaj, iran
نشانی اینترنتی http://www.ijrr.com/browse.php?a_code=A-10-1-222&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Radiation Biology
نوع مقاله منتشر شده تحقیق بدیع
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات