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Iranian Journal of Public Health، جلد ۴۸، شماره ۱۲، صفحات ۲۲۸۵-۲۲۹۲
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عنوان انگلیسی Super Infection of Cutaneous Leishmaniasis Caused by Leish-mania major and L. tropica to Crithidia fasciculata in Shiraz, Iran
چکیده انگلیسی مقاله Abstract Background: The aims of the current study were to determined present status of CL in Shiraz City, identify the causative species of Leishmania and conduct phylogenetic evaluations in detected parasites Methods: This study was conducted on 70 individuals with suspected CL that referred to the major health centers of Shiraz (Valfajr), Fars province, Iran, from Sep 2016 to Jul 2017. DNA was extracted from cultured Leishmania promastigotes and PCR-RFLP were performed using ITS1-rDNA gene. Results: Overall, 39 male (55.70%) and 31 (44.30%) female were found to be positive microscopically. All of direct examined positive samples were confirmed to be positive for Leishmania spp. DNA. Based upon the PCR-RFLP patterns and phylogenetic analysis, 46 (65.72%), 17 (24.28%) and 7 (10%) isolates were clearly identified as L. major, L. tropica and C. fasciculata, respectively. Conclusion: The dominat detected species in Shiraz City was L. major and L. tropica, respectively. CL has high prevalence in Shiraz City; therefore, more studies on leishmaniasis in the natural vectors and also reservoirs infection in this region is exceedingly recommended. Skin leisons due to C. fasciculata, was described for the first time in Iran (Shiraz City).  
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نویسندگان مقاله | Aliyar MIRZAPOUR
Department of Medical Parasitology and Mycology, School of Medicine, Mashhad Branch, Islamic Azad University, Mashhad, Iran


| Alireza BADIRZADEH
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Marzieh ASHRAFMANSOURI
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Hamed BEHNIAFAR
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Maryam NOROUZI
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Hakim AZIZI
Department of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran


| Mahmoodreza BEHRAVAN
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Seyyed Javad SEYYED TABAEI
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://ijph.tums.ac.ir/index.php/ijph/article/view/19084
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