این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Medical Sciences، جلد ۴۵، شماره ۱، صفحات ۵۰-۵۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Cross-Resistance of Acquired Radioresistant Colorectal Cancer Cell Line to gefitinib and regorafenib
چکیده انگلیسی مقاله Background: Usually, chemoradiotherapy can be used for the treatment of locally advanced colorectal cancer (CRC) before surgery. On the other hand, some studies have shown that fractional radiation of tumor cells leads to chemoresistance. The aim of this study was to evaluate the chemoresistance of radioresistant sub-line (RR sub-line).Methods: This study was done in Hamadan University of Medical Sciences in 2017-2018. MTT assay and sub-G1 fraction analysis by flow cytometry were used to evaluate cross-resistance of RR sub-line to gefitinib and regorafenib. Real-time PCR was used to investigate the role of four miRNAs and their target genes in the cross-resistance of RR sub-line. The t test and repeated measures test were used for the assessment of statistical significance between groups.Results: The IC50 of gefitinib and regorafenib for RR sub-line were significantly higher than those of the parental cell line. On the other hand, the resistance index of RR sub-line for gefitinib and regorafenib were 1.92 and 1.44, respectively. The sub-G1 fraction of RR sub-line following treatment with gefitinib and regorafenib was significantly lower than that of the parental cell line (P=0.012 and P=0.038, respectively). The expression of miR-9, Let-7e, and Let-7b in RRsub-line was significantly lower than that of the parental cell line. However, NRAS, IGF1R, NFKB1, and CCND1 found to be upregulated in RR sub-line in comparison with the parental cell line.Conclusion: We can conclude that the acquired RR sub-line was cross-resistance to gefitinib and regorafenib. Furthermore, miR-9/NFKB1, let-7b/CCND1, let-7e/NRAS, and IGF1R played essential roles in the chemoradioresistance of CRC.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله Saeid Afshar |
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Abdolazim Sedighi Pashaki |
Mahdieh Radiotherapy and Brachytherapy Charitable Center, Hamadan, Iran

Rezvan Najafi |
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Safoora Nikzad |
Department of Medical Physics, Hamadan University of Medical Sciences, Hamadan, Iran

Razieh Amini |
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Nooshin Shabab |
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Omid Khiabanchian |
Medical Laboratory, Besat Hospital, Hamadan, Iran

Hamid Tanzadehpanah |
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Massoud Saidijam |
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

نشانی اینترنتی http://ijms.sums.ac.ir/article_44972_ed2b146c37821c09f96ef4ed45f0412d.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/147/article-147-2191103.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات