این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱۱، شماره ۶، صفحات ۴۹۶-۵۰۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Molecular characterization of quinolone resistant Shigella spp. isolates from patients in Ardabil, Iran
چکیده انگلیسی مقاله Background and Objectives: Shigella is an etiological agent of shigellosis. Antibiotic therapy has a critical role in decreasing serious complications of shigellosis. The present study aimed to determine the multi-drug resistance strains and to detect fluoroquinolone related mutations. Materials and Methods: In this descriptive, cross sectional study, a total of 113 Shigella isolates were collected from 1280 patients admitted to Bu-Ali hospital in Ardabil province during 2015-17. Antibiotic resistance pattern of isolates was evaluated using Kirby Bauer method and finally, the MICs of ciprofloxacin were determined. In order to determine any mutations in QRDR region, parC and gyrA genes of resistant strains were amplified and sequenced. Results: Shigella spp. isolates were identified using ipaH amplification and rfc and wbgz genes were used for molecular detection of S. flexneri and S. soneii, respectively. Our results showed that the predominant species in Ardabil province was S. sonnei (69.91%). Most of isolates (82%) were resistant to trimethoprim/sulfamethoxazole (TMP/SMX); 51% were nalidixic acid resistant and 4.4% were floroquinolones resistant. All examined isolates were susceptible to imipenem (100%). Mutation in gyrA and parC genes were detected in all fluoroquinolone resistant isolates (5 isolates). Although, in this study the rate of resistance to ciprofloxacin was low, but in the lack of preventive strategy it will be a major challenge of public health in future. Conclusion: This study provided information on the prevalence and antimicrobial susceptibility patterns of Shigella isolates in Ardabil province, Iran. Also this study showed a high-level of resistance to commonly used antibiotics among Shigella isolates.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Roghayeh Teimourpour
Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran


| Behnam Babapour
Microbiology Laboratory, Bu-Ali Hospital, Ardabil University of Medical Sciences, Ardabil, Iran


| Majid Esmaelizad
Department of Biotechnology, Central Laboratory, Razi Vaccine and Serum Research Institute, Karaj, Iran


| Mohsen Arzanlou
Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran


| Hadi Peeri-Doghaheh
Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/2113
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/91/article-91-2202688.pdf
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات