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Iranian Journal of Basic Medical Sciences، جلد ۲۳، شماره ۳، صفحات ۳۹۰-۳۹۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Effect of rutin on oxidative DNA damage in PC12 neurons cultured in nutrients deprivation condition
چکیده انگلیسی مقاله Objective(s): Rutin is a flavonoid with potent antioxidant property, which exhibited cytoprotective effects in several models of neuronal injury. This work aimed to examine whether rutin can protect neurons against oxidative DNA damage caused by serum/glucose deprivation (SGD) as an in vitro model of neurodegeneration and ischemia. Materials and Methods: The PC12 cells were cultured for 2 hr in normal culture medium containing different concentrations of rutin or α-tocopherol (positive control) and then further incubated for 12 hr in SGD condition. Then, cell viability, DNA fragmentation, lipid peroxidation, generation of reactive oxygen species (ROS), and the expression of proteins involved in apoptosis were determined. Results: The SGD condition significantly decreased viability of the cells, which was accompanied by a significant rise in the generation of ROS and lipid peroxidation. Rutin enhanced the viability of PC12 cells in SGD condition and reduced the production of ROS and lipid peroxidation. In addition, rutin decreased DNA damage and inhibited apoptotic cell death by decreasing the levels of proapoptotic proteins (Bax, caspase-3, caspase-9) and increasing the level of anti-apoptotic protein Bcl-2.Conclusion: This study demonstrated that rutin inhibits oxidative DNA damage and neuronal death induced by nutrients deprivation condition. Further studies may warrant the use of rutin as an appropriate neuroprotective agent for ischemic attacks and other neurodegenerative disorders.
کلیدواژه‌های انگلیسی مقاله Apoptosis, DNA, Oxidative stress, PC12, Rutin
نویسندگان مقاله | Marjan Nassiri-Asl
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran


| Ahmad Ghorbani
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Sahar Salehisar
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Elham Asadpour
Anesthesiology and Critical Care Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| Hamid Reza Sadeghnia
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran|Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad, University of Medical Sciences, Mashhad, Iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_14632.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-2230152.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
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