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Acta Medica Iranica، جلد ۵۷، شماره ۸، صفحات ۴۷۲-۴۷۷
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عنوان انگلیسی Epigenetic inactivation of protocadherin 10 by methylation in colorectal cancer
چکیده انگلیسی مقاله Aberrant promoter methylation of CpG islands of tumor-suppressor genes has been recognized as one of the important tumor markers for cancer detection. The aim of this study was to investigate the promoter methylation status of protocadherin 10 (PCDH10), a tumor suppressor gene, in Iranian CRC patients. Cancerous and the adjacent normal tissues obtained from 38 CRC patients were used to assess methylation status of PCDH10 with Methylation Specific PCR in addition to study the expression level of this gene by quantitative PCR. The relationship between hypermethylation and demographic characteristics of these patients was analyzed. Promoter methylation level of PCDH10 was statistically different between tumoral and normal tissues in CRC patients. Twenty seven out of 38 patients showed hypermethylation with a sensitivity of 73% and a specificity of 97%. PCDH10 expression decreased in 15 cases (46%) as 16 cases (50 %) showed over- expression and 1 case (4%) had no changes. Not a significant association was reported between PCDH10 hypermethylation and the clinicopathological characteristics (p value>0.05). Our results indicated that PCDH10 methylation has critical function in CRC, with a nearly elevated sensitivity and a high specificity in Iranian population, qualify it as a potential candidatebiomarker.
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نویسندگان مقاله | Mohammad Amin Kerachian
Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
 
 Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
 
 Cancer Genetics Research Unit, Reza Radiotherapy and Oncology Center, Mashhad, Iran


| Sahar Tavakolian
Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
 
 Department of Biology, University of Sistan and Balouchestan, Zahedan, Iran


| Matineh Barati Bagherabad
Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
 
 Department of Biology, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran


| Dor Mohammad Kordi-Tamandani
Department of Biology, University of Sistan and Balouchestan, Zahedan, Iran


| Mohammad Reza Abbaszadegan
Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
 
 Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
 
 Division of Human Genetics, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
نشانی اینترنتی http://acta.tums.ac.ir/index.php/acta/article/view/7640
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/56/article-56-2281800.pdf
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