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Iranian Journal of Basic Medical Sciences، جلد ۲۳، شماره ۴، صفحات ۵۰۷-۵۱۴
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عنوان انگلیسی Induction of humoral immune responses and inhibition of metastasis in mice by a VEGF peptide-based vaccine
چکیده انگلیسی مقاله Objective(s): Blocking of vascular endothelial growth factor (VEGF) plays a pivotal role in inhibition of metastasis and is a target for development of anti-angiogenic agents. In this study, a peptide-based vaccine was designed and its potential for induction of humoral immune responses, generation of neutralizing antibodies, inhibition of tumor growth and metastasis was determined.Materials and Methods: With online bioinformatics tools, a fragment of the VEGF-A was selected for a peptide-based vaccine. To enhance its antigenicity, the peptide was conjugated with Keyhole limpet hemocyanin and used to immunize mice. Then, the polyclonal anti-VEGF antibody titer was measured and its effect on proliferation of HUVEC cell line was investigated by MTT assay. Finally, we checked the effect of the peptide on tumor growth, metastasis, and survival rates in a mouse model of cancer.Results: The bioinformatics analysis of the selected region confirmed dis-similarity of the peptide with any other human protein and its acceptable antigenicity to stimulate a tumor-specific humoral response. Anti-VEGF antibody titers were significantly greater in vaccinated mice than in controls. IgG antibody from mice immunized with recombinant VEGF-A inhibited HUVEC proliferation (P
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نویسندگان مقاله | Faezeh Soltanpour Gharibdousti
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran|Immunology Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Banafsheh Fazeli Delshad
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran|Immunology Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Reza Falak
Immunology Research Center, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Nasrin Shayanfar
Department of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Mazdak Ganjalikhani Hakemi
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran


| Alireza Andalib
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran


| Gholamali Kardar
Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_14629.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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